BioDynami offers targeted sequencing service to our customers who prefer complete sample-to-data service. Our expertise with the CATCH-Seq technology process and our automation of many of the steps in the CATCH-Seq protocol lead to a rapid turnaround time for your project. We have experience with projects large and small, so whatever your project needs, BioDynami can supply you with high quality targeted sequencing service. In many cases, we can deliver better data in less time than a local core facility.

Targeted Sequencing Service

MHC sequencing: Capture and sequence the human MHC locus including exons, introns, and flanking sequences.

LRC/KIR sequencing: Capture and sequence the human KIR locus including exons, introns, and flanking sequences.

Breast cancer panel: Capture and sequence coding and non-coding regions of up to 117 human genes related to breast cancer.

Custom CATCH-Seq targeted sequencing: Capture and sequence the customized large target regions of 200 kb to >10 Mb.

Sample requirements and output specs

• DNA requirement: 500 ng
• On-target rate: 70-80%
• Mean coverage: 80-100X
• Total bases covered: >85%

To get a quote, email us at sales@biodynami.com, or contact us here.

Also, please check the Sample Submission Guideline before submitting your samples to us.

Targeted Sequencing Products

MHC targeted sequencing kits: Capture the human MHC locus including exons, introns, and flanking sequences.

LRC/KIR targeted sequencing kits: Capture ~1.3 Mb of the human KIR locus including exons, introns, and flanking sequences.

Breast cancer panel targeted sequencing kits: Capture coding and non-coding regions of 117 breast cancer related genes.

MHC Sequencing Service

MHC:
~3.8 MB of human MHC loci, including Class I, Class II, and Class III

MHC Class I:
~2.1 MB of complete human MHC Class I loci

MHC Class II:
~0.7 MB of complete human MHC Class II loci

MHC Class III:
~1.1 MB of complete human MHC Class III loci

MHC Core:
~190 KB of 22 human MHC genes, including exons, introns, and 5′ and 3′ flanking regions

MHC Targeted Sequencing Service

		MHC Full	MHC Class I	MHC Class II	MHC Class III	MHC Core
Target size		3.8 MB	2.1 MB	0.7 MB	1.1 MB	190 KB
Service	library prep	included	included	included	included	included
	capture	included	included	included	included	included
	mean coverage	80-100X	80-100X	80-100X	80-100X	80-100X
Cost	24 samples	$310 per sample	$290 per sample	$275 per sample	$280 per sample	$285 per sample
	96 samples	$300 per sample	$249 per sample	$234 per sample	$239 per sample	$228 per sample
	384 samples	$290 per sample	$239 per sample	$212 per sample	$217 per sample	$189 per sample

LRC/KIR Sequencing Service

Full human LRC/KIR region

Covers 1.3 MB region
Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond

Easy detection of SNPs, indels, and structural variants

The only reagent provides intact sequence information

Service	library prep	included
	capture	included
	mean coverage	80-100X
Cost	24 samples	$290 per sample
	96 samples	$249 per sample
	384 samples	$227 per sample

Breast Cancer Panel Sequencing Service

Breast Cancer Panel
The Panel covers ~9 MB of target space of the 117 human breast cancer related genes.

Breast Cancer Twenty Genes SubPanel
choose custom subsets of 20 genes from within the 117 gene Panel at a lower cost than the full panel.

Breast Cancer Ten Genes SubPanel
choose custom subsets of 10 genes from within the 117 gene Panel at a lower cost than the full panel.

Breast Cancer Panel Selection

		Full Panel	Ten Genes SubPanel	Twenty Genes SubPanel
Gene set		117 genes	customized 20 genes	customized 10 genes
Service	library prep	included	included	included
	capture	included	included	included
	mean coverage	80-100X	80-100X	80-100X
Cost	24 samples	$500 per sample	$330 per sample	$300 per sample
	96 samples	$475 per sample	$289 per sample	$259 per sample
	384 samples	$450 per sample	$255 per sample	$225 per sample

Gene List of Breast Cancer Panel

ABCB1	BRCA2	DYNLRB1	FOXA1	IGFBP1	MAPK3	N4BP2L1	PIK3CA	SEC16A	TP53
ABCG2	CCND1	EGF	FUS	IGFBP3	MAPK8	NBR2	PIK3R1	SERPINE1	TRIM46
AKT1	CCND2	EGFR	GATA3	IL6	MDM2	NEK2	PLAU	SFN	TUSC2
APC	CDH1	EP300	GDPD3	INPP5E	MGMT	NME1	PNMT	SFRP1	TWIST1
AR	CDK2	ERBB2	GLI1	ITCH	MINE1	NME2	PPP1R1B	SNAI2	VEGFA
ARHGAP9	CDKN1A	ERBB2	GPR137	JUN	MKI67	NOTCH1	PTEN	SRC	WEE1
ATM	CDKN1C	ERBB3	GRB7	KLLN	MLH1	NPRL2	PTGS2	STARD3	XBP1
BAD	CDKN2A	ESR1	GSTP1	KRT5	MMP2	NR3C1	PYCARD	TCAP	ZAR1L
BAP1	CSF1	ESR2	HIC1	LPAR6	MMP9	OVCA2	RASSF1	TFF3	ZMYND10
BCL2	CTNNB1	FBXO32	ID1	MAP1LC3A	MUC1	PA2G4	RB1	TGFB1
BIRC5	CTSD	FGFR1	IGF1	MAP3K1	MUC16	PGAP3	RET	THBS1
BRCA1	CYB561D2	FGFR2	IGF1R	MAPK1	MYC	PGR	RPL41	THBS3

Custom CATCH-Seq targeted sequencing: Choose your own target regions

Large target region

Covers several hundred kilobases to several megabases of human contiguous sequence.
Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond.

Multiple target regions possible

Single or multiple target regions can be captured.

Sample multiplexing

Allows you to multiplex samples in one NGS run to further reduce your cost.

Low cost

We provide the lowest price for capture reagent with high quality.

Example of Custom CATCH-Seq Targeted Sequencing: Four gene in >200 kb region

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