CATCH-Seq Technology

The proprietary CATCH-Seq targeted sequencing technology leverages genomic clones to generate probes corresponding to large, contiguous blocks of a genome. This is particularly useful when your research calls for sequencing coding and non-coding regions of a gene, including introns and flanking sequences.  Sequencing large target regions including introns make it ideal for identification of structural variants and CNV’s.

CATCH-Seq target enrichment is also ideal for studies of DNA methylation, by performing targeted bisulfite sequencing after the target enrichment steps.  CATCH-Seq target enrichment technology is compatible with any NGS library or platform.  Best of all, since CATCH-Seq technology requires no oligonucleotide design and production costs, custom capture reagents are available at a fraction of the cost of other target enrichment methods.

Advantages of CATCH-Seq

Large target regions: CATCH-Seq capture probes can cover several hundred kilobases to several megabases of contiguous sequence and can cover exons, introns, 5’ regulatory regions, 3’ regulatory regions, and flanking regions.

Greater specificity with longer probes (200-300bp)

Better coverage of the targeted region including repeats

Significantly lower cost than oligo-based probes

Multiplexed capture of samples reduces costs