Urine Cell-free DNA Extraction Kit (Magnetic Beads)
The Urine Cell-free DNA Extraction Kit (Magnetic Beads) is developed for the efficient extraction of cell-free DNA (cfDNA) from fresh or frozen urine samples. Urinary cfDNA carries valuable genetic and epigenetic information, making it a powerful tool for biomarker discovery in cancer and other disease research.
Urine offers two key advantages as a source for biomarker discovery: First, larger sample volumes can be obtained compared to plasma or serum. Second, urine collection is truly non-invasive. Additionally, the same individual can provide repeated samples over time, enabling longitudinal studies.
Urinary cfDNA has been increasingly studied for the diagnosis and prognosis of various cancers and diseases. However, sample composition can vary significantly between individuals due to factors such as cellular heterogeneity, health and disease status, stress levels, medical treatments, and more.
DNA in urine is primarily derived from cells shed from the urinary tract. During apoptosis and necrosis, chromosomal DNA is fragmented—a process that is more pronounced under pathological conditions including cancer development, inflammation, and immune responses. As a result, urine cfDNA is typically highly degraded, with fragments around 100 bp or shorter.
Samples of extracted DNA from urine.
Our kit leverages Solid Phase Reversible Immobilization (SPRI) technology—a simple, bead-based method ideally suited for recovering cfDNA from both fresh and frozen urine samples. The protocol eliminates tedious steps such as spin column centrifugation and vacuum filtration, offering a reliable and convenient workflow for isolating high-quality cfDNA.
Recovered DNA fragments can be eluted in volumes as low as 10 µl, enabling high-concentration yields for sensitive applications. The extracted cfDNA is fully compatible with a wide range of downstream techniques, including bisulfite sequencing, next-generation sequencing (NGS), microarray, PCR, qPCR, and more.
Two kit formats are available to accommodate different urine input volumes: 5 ml (Cat. # 50103) and 40 ml (Cat. # 50104).
Specifications
Specifications | |
Technology | Magnetic beads |
Sample type | Fresh or frozen urine samples |
Sample volume | 5 ml (Cat. # 50103) or 40 ml (Cat. # 50104) |
Purity | cfDNA is NGS and PCR ready. Typical A260/A280 is around 1.8. Note: sample quantification or purity assessment using a spectrophotometer is not recommended due to the limited amount of DNA. |
Yield range | Typical yield: 0.5 ng to 20 ng from healthy donors. |
Fragment sizes | From 60 bp to >10 kb genomic DNA. |
Elution volume | As low as 10 µl is OK. |
Features
- cfDNA isolation from fresh and frozen urine samples
- Simple beads-based technology
- No column required
- No vacuum required
- Low elution volume: 10 µl is OK
- Removal of impurities
Protocol & MSDS Download
Product Category
Kit Components
- Cat.# 50103S/50103L: 10/40 rxns (5 ml sample volume)
- Cat.# 50104S/50104L: 10/40 rxns (40 ml sample volume)
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Background info