Plasma/Serum Cell-free DNA/RNA Extraction Kit (Magnetic Beads)
The Plasma/Serum Cell-free DNA/RNA Extraction Kit (Magnetic Beads) is developed for the efficient isolation of cell-free DNA (cfDNA) and cell-free RNA (cfRNA) from fresh or frozen plasma and serum samples.
The analysis of cfDNA and cfRNA in these biofluids is a powerful tool for disease diagnosis, particularly in oncology. These nucleic acid fragments, released into the bloodstream during normal physiological processes such as apoptosis, as well as from necrotic cancer cells, hold immense clinical value as non-invasive biomarkers. Liquid biopsy using cfDNA/RNA is now a well-established method for both cancer diagnosis and prognosis, enabling the detection and monitoring of disease through a simple blood draw.
cfDNA fragments are typically short, averaging around 170 base pairs (bp), which corresponds to the length of DNA wrapped around a mononucleosome. The fragmentation pattern of DNA in circulation can be influenced by various pathological conditions, including cancer development, infection, immune reactions, and inflammation, further enhancing its diagnostic potential.
Samples of extracted DNA from plasma.
Our kit leverages proprietary Solid Phase Reversible Immobilization (SPRI) magnetic bead technology, optimized specifically for the superior recovery of minute quantities of cfDNA and cfRNA from cell-free fluids. The streamlined protocol eliminates the need for time-consuming centrifugation or vacuum steps. Purified fragments can be eluted in volumes as low as 10 µl, maximizing concentration for sensitive downstream assays.
The extracted, high-quality cfDNA and cfRNA are fully compatible with a wide range of demanding applications, including next-generation sequencing (NGS), bisulfite sequencing, PCR, and qPCR.
Specifications
Specifications | |
Technology | Magnetic beads |
Sample type | Fresh or frozen plasma/serum |
Sample volume | 1 ml |
Purity | cfDNA/RNA is NGS and PCR ready. Typical A260/A280 is around 1.8. Note: sample quantification or purity assessment using a spectrophotometer is not recommended due to the limited amount of DNA/RNA. |
Yield range | Typical yield: 0.5 ng to 20 ng from healthy donors. |
Fragment sizes | From 100 bp to >10 kb genomic DNA and RNA. |
Elution volume | As low as 10 µl is OK. |
Features
- High-quality cfDNA and cfRNA extraction from plasma and serum
- Simple beads-based technology
- No column needed
- No vacuum needed
- Elution volume as low as 10 µl
- Removal of unwanted components and other impurities
Protocol & MSDS Download
Product Category
Kit Components
- Cat.# 50102S/50102L: 10/40 rxns
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Background info