Bacterial tRNA Extraction Kit (Magnetic Beads)
The Bacterial tRNA Extraction Kit (Magnetic Beads) enables efficient microbial tRNA extraction from gram-negative and gram-positive bacteria, fungi, and yeast using advanced magnetic bead technology. The procedure is fast and reliable, yielding high-quality tRNA free from contamination by larger RNA species such as mRNA, 16S rRNA, and 23S rRNA.
The kit employs a sequential purification process: genomic DNA is removed first, followed by the elimination of large RNA (mRNA, 16S rRNA, and 23S rRNA), after which tRNA is purified and eluted.
Microorganism samples are initially lysed using a lysis buffer combined with the first beads. Genomic DNA binds to these beads and is discarded, while the supernatant containing both large RNA and tRNA proceeds to the next step. This supernatant is then mixed with the second beads, which selectively bind to large RNA but not tRNA. The large RNA bound to these beads is discarded, leaving a supernatant enriched with tRNA. Finally, this supernatant is mixed with the third beads, which bind and purify the tRNA before elution.
The kit is free of toxic substances such as phenol and chloroform and allows for simultaneous processing of multiple samples.
Purified tRNA is free of genomic DNA, large RNA (mRNA, 16S rRNA, and 23S rRNA), and other contaminants including salts, lipids, and proteins. tRNA extracted using this kit is suitable for various downstream applications, including next-generation sequencing (NGS), hybridization, qPCR, and RT-PCR.
Three samples were loaded on agarose gel. Left panel: both the large RNA fraction and tRNA fraction are shown on a 1% agarose gel to demonstrate the separation of the tRNA fraction from the large RNA fraction. Right panel: the tRNA fraction is shown on a 3% agarose gel. A small amount of 5S rRNA is present in the samples due to its small size, similar to tRNA.
Specifications
Specifications | |
Technology | Magnetic beads |
Starting volume | 0.4 – 2 ml (up to 10^9 bacteria cells) |
Starting material | Gram-positive; gram-negative; yeast; fungi |
Elution volume | 10-50 µl |
O.D. 260/280 | Around 2.0 |
O.D. 260/230 | 1.6- 2.0 |
Features
- High-quality tRNA extraction
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- Without large RNA (mRNA, 16S rRNA, 23S rRNA etc.)
- Free of impurities/contaminations
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- Simple protocol
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- No column
- No vacuum
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- Safe procedure
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- No phenol
- No chloroform
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- No additional DNase treatment needed.
Protocol & MSDS Download
Product Category
Cat.# & Rxns
- Cat.# 50342S: 10 rxns
- Cat.# 50342L: 50 rxns
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Background info