RNase A (Ribonuclease A) Cleavage Principle

RNase A (Molecular Biology Grade, DNase-free), 10 mg/ml

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Description

RNase A (Ribonuclease A), 10 mg/ml

RNase A (ribonuclease A), isolated from bovine pancreas, is an enzyme composed of 124 amino acids with a molecular weight of 13.7 kDa. RNase A is an endonuclease that specifically cleaves the phosphodiester bonds in RNA molecules at the 3′ end of pyrimidine residues (cytosine and uracil). The optimal pH is around 7.0 to 8.0.

The RNA cleavage specificity may be different depending on the salt concentration. In the cases of low salt concentrations (for example, under 0.1 M of NaCl), RNase A cleaves both single-stranded RNA and double-stranded RNA, as well as the RNA molecule in RNA/DNA hybrids. In cases of high salt concentrations (for example, NaCl concentrations over 0.3 M), RNase A specifically cleaves single-stranded RNA.

RNase A (Ribonuclease A) Cleavage Principle

RNase A can be used for genomic DNA and plasmid extraction, the removal of non-hybridized regions of RNA/DNA hybrids, degradation of unwanted RNA in gene expression analysis, and RNase protection assays.

RNase A is very stable and hard to denature and degrade. The enzyme can be inhibited by RNase inhibitors and several chemicals, such as diethyl pyrocarbonate (DEPC), guanidinium thiocyanate (GITC), SDS, and vanadyl ribonucleoside complex (VRC).

Specification

      • Concentration: 10 mg/ml
      • Grade: Molecular biology grade
      • Source: Bovine pancreas
      • Molecular weight: 13.7 kDa
      • Shipping: At room temperature. Upon receipt, store at -20°C.
      • Storage: -20°C. Stable up to 12 months.
      • Storage Buffer: 50 mM Tris-HCl (pH 7.5), 50% glycerol
      • Optimal pH: 7.0 – 8.0
      • Optimal enzymatic temperature: 37°C
      • Activity: ≥ 50 Kunitz units/mg
      • DNase Contamination: Not detected
      • Proteinase Contamination: Not detected

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Inhibition and Inactivation

RNase inhibitors, guanidinium thiocyanate (GITC), diethyl pyrocarbonate (DEPC), sodium dodecyl sulfate (SDS), vanadyl ribonucleoside complex (VRC), 5′-diphosphoadenosine 3′-phosphate (ppA-3′-p), diphosphoadenosine 2′-phosphate (ppA-2′-p), and heavy metals. RNase A can be inactivated by phenol/chloroform extraction.

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Applications

      • RNA removal from genomic DNA and plasmid DNA extraction
      • RNA removal from protein extraction
      • Degradation of unwanted RNA in gene expression analysis
      • Ribonuclease related assays

 

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Contents

RNase A (10 mg/ml)

Cat.# 40106S: 1 ml (10 mg/ml)
Cat.# 40106L: 5 ml (10 mg/ml)

Protocol & MSDS

RNase A, 10 mg/ml  (Cat.# 40106S; 40106L)

Protocol download
MSDS download

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