Genomic DNA Extraction Kit for Plants (HMW, Magnetic Beads)
The Genomic DNA Extraction Kit for Plants (HMW, Magnetic Beads) is a fast and simple reagent for isolating high molecular weight (HMW) genomic DNA from plants using Solid Phase Reversible Immobilization (SPRI) magnetic beads. The magnetic beads-based kit provides a consistent and reliable approach for high-quality plant DNA extraction due to the high-binding capacity and quick magnetic response time.
The extracted plant DNA sizes are above 50 kb and the size ranges are between 50-200 kb. The kit is developed to minimize the common contamination of polysaccharides and polyphenols in plant DNA samples. Purified plant genomic DNA is recovered at high yield and high purity without RNA, polysaccharides, polyphenols, lipids, and salt contaminations.
The extracted extracted genomic DNA samples from various plants were loaded on a PFGE gel.
The ratios of A260/A280 are around 1.8, and the ratios of A260/A230 are around 1.5-2.5. Purified HMW plant DNA is suitable for applications such as, long range PCR, restriction enzyme digestion, hybridization assays, long-read sequencing, and other applications.
Sample Input | DNA Yield by Qubit | DNA Yield by Nanodrop | ||||
Plant | Parts | Amount | BioDynami | Company O | BioDynami | Company O |
spinach | leaf | 20 mg | 1.78 µg | 0.54 µg | 3.32 µg | 1.13 µg |
cilantro | leaf | 20 mg | 1.43 µg | 0.55 µg | 2.87 µg | 1.14 µg |
holly | leaf | 10 mg | 0.48 µg | 0.15 µg | 0.71 µg | 0.36 µg |
pothos | leaf | 40 mg | 0.56 µg | 0.12 µg | 0.65 µg | 0.31 µg |
garlic | root | 35 mg | 8.06 µg | 1.78 µg | 8.63 µg | 2.35 µg |
apple | seed | 50 mg | 0.83 µg | 0.49 µg | 2.58 µg | 1.39 µg |
pepper | seed | 25 mg | 3.63 µg | 0.41 µg | 4.82 µg | 0.97 µg |
Yield comparison of plant genomic DNA extraction. Kits from BioDynami and Company O were used. DNA samples are quantified using Qubit fluorometer and Nanodrop. Plant species, parts and amounts are indicated. Total amount of extracted genomic DNA are listed.
Plant tissues, such as fresh leaves, seeds, pedals, roots, are homogenized and lysed, then magnetic beads is added to bind plant DNA before the supernatant is removed by magnetic separation. The DNA-binding magnetic beads are washed to remove residual polysaccharides and polyphenols, then resuspend in a buffer followed by magnetic separation to remove supernatant from the DNA-binding beads. The Purified plant DNA is eluted in the Elution Buffer after the washing steps.
Plant | Parts | O.D. 260/280 Comparison | O.D. 260/230 Comparison | ||
BioDynami | Company O | BioDynami | Company O | ||
spinach | leaf | 1.96 | 1.73 | 2.05 | 0.98 |
cilantro | leaf | 1.86 | 1.62 | 2.01 | 0.67 |
holly | leaf | 1.64 | 1.92 | 2.16 | 0.72 |
pothos | leaf | 1.71 | 1.44 | 2.18 | 0.22 |
garlic | root | 1.73 | 1.69 | 2.19 | 1.52 |
apple | seed | 1.89 | 1.72 | 1.94 | 1.53. |
pepper | seed | 1.77 | 1.65 | 1.28 | 1.03 |
Comparison of O.D. 260/280 and O.D. 260/230 from extracted plant DNA samples. The values are based on the data from Nanodrop.
Specifications
Specifications | |
Technology | Magnetic beads |
Tissue amount | Up to 50 mg |
Genomic DNA size | 50-200 kb |
Genomic DNA yield | Up to 10 µg (dependent on the plant) |
Elution volume | 100 µl |
O.D. 260/280 | Around 1.8 |
O.D. 260/230 | Between 1.5-2.5. May have exception. |
Features
- High molecular weight DNA: 50 kb to 200 kb
- Unique magnetic beads technology
- No vacuum needed
- No column needed
Protocol & MSDS Download
Kit Components
- Cat.# 50021S/50021L: 50/200 rxns
- Cat.# 50021SP/50021LP: 50/200 rxns (with Pestle Set)
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