RNA Seq Library Prep Kit (illumina)

RNA Seq Library Prep Kit

NGS DNA Library Prep Kit (Ion Torrent Platform)NGS Library Quantification Standards (Ion Torrent)

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Description

RNA Seq Library Prep Kit (illumina and MGI Platforms)

RNA Seq Library Prep Kit (illumina)

The RNA Seq Library Prep Kit was developed for construction of high quality NGS  libraries for next generation sequencing (illumina and MGI Platforms). RNA Sequencing is a very powerful tool to analyze transcriptome such as gene expression and transcription regulation, splicing characterization, mutation and variation detection etc. The kit needs purified RNA (example: rRNA depleted RNA or polyA mRNA) as input for library construction. Library multiplexing is possible with different types of indexes.

 

RNA Seq Library Prep Kit (illumina)

RNA Seq Library Prep Kit Workflow

Three index types are available for the illumina platform kits:

Non-index (illumina Cat.# 30055): Libraries do not have index.

Index (illumina Cat.# 30056): A unique barcode sequence with 6 bases has been included in each of the index primers. RNA Sequencing library multiplexing is possible with up to 48 samples. Index information can be downloaded here.

Unique dual index (illumina Cat.# 30057): RNA Sequencing library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. the unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34112).

Features

      • Quick protocol
        • Libraries will be ready in 2 hours
        • Hands-on time is only ~10 minutes
      • Guaranteed quality
        • High yield
        • Uniform coverage of transcripts
      • Simple workflow
      • Input purified RNA amount: From 3 ng to 100 ng

 

RNA Seq Library Prep Kit

Comparison of the protocol time: BioDynami RNA Seq Library Prep Kit vs other vendors’ kits. Hands-on time and walk-away time were indicated.

 

RNA Seq Library Prep Kit - distribution

Library size and distribution of BioDynami kit, 20 ng and 3 ng of polyA mRNA as input.

 

RNA Seq Library Prep Kit - yield and duplication

Comparison of library yield and duplication rate under the same condition: 20 ng and 3 ng of polyA mRNA were used as input. PCR cycle numbers were indicated.

 

RNA Seq Library Prep Kit - coverage

Comparison of coverage of transcripts: 20 ng of polyA mRNA were used as input. BioDynami kit has more uniform coverage.

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Contents

RNA Seq Library Prep Kit (illumina Platform, Cat.# 30055)

RS1 Buffer
RS1 Enzyme
RS2 Enzyme
RS3 Buffer
RS3 Enzyme
Non-index Primers
PCR mix

RNA Seq Library Prep Kit (illumina Platform, Cat.# 30056)

RS1 Buffer
RS1 Enzyme
RS2 Enzyme
RS3 Buffer
RS3 Enzyme
Index Primers
PCR mix

RNA Seq Library Prep Kit (illumina Platform, Cat.# 30057)

RS1 Buffer
RS1 Enzyme
RS2 Enzyme
RS3 Buffer
RS3 Enzyme
Unique Dual Index Primers
PCR mix

RNA Seq Library Prep Kit (MGI Platform, Cat.# 34056)

RS1 Buffer
RS1 Enzyme
RS2 Enzyme
RS3M Buffer
RS3 Enzyme
Index Primers
PCR mix

Protocol & MSDS

RNA Seq Library Prep Kit (illumina Platform, Cat.# 30055, 30056, 30057)

Protocol download
MSDS download

 

RNA Seq Library Prep Kit (MGI Platform, Cat.# 34056)

Protocol download
MSDS download

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