Description

NGS DNA Library Prep Customization Kit

The kit was developed for construction of high quality libraries for next generation sequencing. The kit is derived from our NGS DNA Library Prep Kit.  Similarly, the kit needs DNA fragments as input for library construction, and is compatible with DNA fragments generated from enzymatic methods and physical methods. Both enzymatic DNA fragmentation (DNA Fragmentation & A-tailing Enzyme Mix Cat.# 40062) and physical DNA fragmentation (sonication etc) have great outcome with the customization kit.

With the expansion of the NGS applications, requirements for library preparation are very diverse and in some cases more customized library prep is needed. To facilitate the DNA library prep customization, we made the kit to help the scientists so they can totally customize the form of their own NGS libraries. Our kit allows the scientists to use their own designed adaptors, PCR primers, and PCR reagents.

Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.

Features

• • Fast
    • Hands-on time: ~4 min
    • Total time: <1 hrs
  • Simple workflow: Two reaction steps for library construction
  • Higher library conversion efficiency
  • Input DNA amount from 100 ng to 1 ug

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