Proteinase K, 20 mg/ml (Recombinant, PCR Grade)
Proteinase K is a serine protease that exhibits broad protein cleavage activity. It is well-used for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid.
The recombinant enzyme has higher proteolytic activity in the presence of SDS and urea, and also has high activity and works in a wide range of pH and temperature. The recommended working concentration is 50–100 μg/ml.
Features of Proteinase K
- Ready-to-use solution format
- Optimal activity between 20 and 60°C. High activity between 50 to 55°C.
- Optimal activity in a pH range of 4.5-12.0; High activity in the pH range of 7.5-11.5.
- No detectable DNase, RNase, endonuclease, and exonuclease activities.
Applications
- Inactivation of DNases, RNases and other enzymes in reactions
- Removal of DNases and RNases for extraction of genomic DNA and RNA from tissues or cells
- Plasmid DNA extraction
- Enhancement of PCR fragment cloning efficiency
- PCR purification
Protocol & MSDS Download
Cat.# & volume of Proteinase K
- Cat.# 40109S: 1 ml
- Cat.# 40109L: 5 ml
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