Plasmid MiniPrep Kits (Magnetic Beads)

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Description

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)

Plasmid MiniPrep Kit (Magnetic Beads)

The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.


Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012
– Use 2 ml of bacteria culture directly

Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011
– High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates
– Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes


The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.

The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.

Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.

 

Plasmid MiniPrep Kit

Plasmid DNA were loaded on 1% of agarose gel after extraction.

Plasmid MiniPrep Kit (Magnetic Beads)

Comparison of workflows of Magnetic Beads based kits

Features

  • 100% centrifuge-free
      • Culture medium can be used directly without centrifuge to pellet the bacteria
  • Simple
      • No centrifuge
      • No column
      • No vacuum
  • Flexible applications with simplified miniprep protocol
      • High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
      • Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
      • Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
      • Ideal for screening and other applications

 

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Contents

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads) (Cat.# 50011)

PS Buffer
NR Buffer
BB Buffer
Elution Buffer

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads) (Cat.# 50012)

PD Buffer
NM Buffer
BM Buffer
Elution Buffer

Protocol & MSDS

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads) (Cat.# 50011)

Protocol download
MSDS download

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads) (Cat.# 50012)

Protocol download
MSDS download