Description
One-Step RT-qPCR Kit
There are two types of kits that can be selected:
• Dye-Based: Kit with an intercalating fluorescent dye allows for DNA detection and analysis without using sequence-specific probes.
• Probe-Based: Kit for DNA detection and analysis using sequence-specific probes.
One-Step RT-qPCR Kit is a fast and convenient solution for performing real-time PCR. The kit combines reverse transcription and qPCR into one reaction, which eliminates the need for two step reactions with the additional transfer samples between the reverse transcription and qPCR steps, thus minimizing sample handling and reducing the risk of contamination.
The proprietary buffer components facilitate the melting of secondary structures of nucleic acids and ensure ultimate complete cDNA synthesis and specificity of the PCR product in just 1.5 hours. The unique formulation of both buffer and enzyme mix delivers consistent performance, and reduces cost and setup time. The kit is compatible with both SYBR-Green and probe-based assays. A unique DNA intercalating dye is used to replace SYBR Green I, but with less PCR inhibition. The reference dye ROX is included for qPCR normalization when needed, making the kit compatible with all real-time PCR instruments.
One-Step RT-qPCR Kit has wide dynamic range, superior sensitivity, specificity, and reproducibility. The kit can be used for both SYBR-Green and probe-based qPCR assays, and are ideal for both low-throughput and high-throughput real-time PCR screening and validation.
One-Step RT-qPCR Kit (Dye-Based) data: Human GAPDH over a 5-log range of input concentrations (20 ng – 2 pg cDNA) with triplicates at each concentration.
One-Step RT-qPCR Kit (Probe-Based) data: Human beta-actin over a 5-log range of input concentrations (50 ng – 5 pg cDNA) with triplicates at each concentration.
Features
- Fast and versatile RT-qPCR compatible with both SYBR-Green or probe-based assays
- Efficient cDNA synthesis coupled with robust qPCR
- Superior sensitivity, specificity, and reproducibility
- Compatible with all real-time PCR instruments – reference dye ROX is included
- Unique buffer and enzyme formulation delivers consistent performance, saving costs and time.
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