NGS Single Stranded DNA Library Prep Kit
The NGS Single Stranded DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using sheared single stranded DNA (50 ng – 500 ng) as input. The kit is ideal for making NGS libraries with samples of denatured DNA, viral DNA, highly degraded ancient DNA and other single stranded DNA. The workflow of the ssDNA kit is simple: make the libraries in two steps followed by PCR and cleanup steps. The libraries will be ready in just 1.5 hours with a 10 minutes of hands-on time. The library pooling is possible dependent on the type of index. The final library is strand specific.
NGS Single Stranded DNA Library Prep Kit Workflow
Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
NGS Single Stranded DNA Library Prep Kit has similar library conversion efficiency and yield as compared to a regular DNA library prep kit.
Alignment rate and duplication rate: comparison of single stranded DNA library kit versus double stranded DNA library prep kit.
Features
- Quick and simple Protocol
- Total protocol time is only 1.5 hours
- Hands-on time only 10 minutes
- Less magnetic beads needed: Reduced more than 50% of the beads for cleanup steps
- Directional library
- Single stranded DNA as input: From 50 ng to 500 ng
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 30083A/30083F/30083S/30083L: 24/48/96/192 rxns
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