NGS DNA Fragmentation & Library Prep Kit (illumina & MGI Platforms)

The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.

NGS DNA Fragmentation & Library Prep Kit Workflow

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.

The library size is inversely correlated with the incubation time of step 1 at 20°C.

Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.

Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34028).

NGS data comparison: enzymatic shearing versus mechanical shearing

Features

• 1.5-hour protocol from intact genomic DNA to NGS library
• Intact genomic DNA as input, DNA fragmentation is not needed.
• Works with both EDTA-free DNA and DNA resuspended in TE buffer
• Simple workflow: Less steps
• Less magnetic beads required for cleanup steps: Save more than 50%

Protocol & MSDS Download

• Protocol download
• MSDS download

Kit Components

illumina Platform	MGI Platform
AD1 Enzyme AD2 Buffer AD2 Enzyme EF Buffer Unique Dual Index Primers PCR mix	AD1 Enzyme AD2m Buffer AD2 Enzyme EF Buffer Index Primers PCR mix

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