Milk DNA Extraction Kit (Magnetic Beads)
The Milk DNA Extraction Kit (Magnetic Beads) was developed for the extraction of nucleic acids from various sources in milk, including bacteria (both gram-negative and gram-positive), fungi, yeast, and host cells. It is also suitable for extracting DNA from mastitic milk samples. This magnetic bead-based kit offers a simple and reliable method for DNA extraction from milk samples, eliminating the need for tedious centrifugation steps.
Kit Workflow
The process begins with enzymatic digestion and lysis buffer, which breaks down bacteria, yeast, fungi, and host cells. Magnetic beads are then added to bind the DNA released from the lysed cells. After the initial DNA separation from the beads, a second bead-binding step is performed to remove additional impurities. Finally, the DNA is washed with ethanol and can be eluted in either elution buffer or TE buffer.
The standard input volume for milk is 500 µl. The yield of milk DNA extraction may vary depending on several factors, such as the bacterial species, type of milk, and cell density. The extracted DNA is suitable for a wide range of downstream applications, including PCR, qPCR, hybridization, restriction digestion, DNA sequencing, and Next-Generation Sequencing (NGS).
DNA from milk samples were isolated and loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb DNA Ladder.
Specifications
| Specifications | |
| Technology | Magnetic beads |
| Milk input volume | 500 µl |
| DNA extraction | Bacteria (gram-positive & gram-negative), yeast, fungi, host cells |
| Elution volume | >20 µl |
| O.D. 260/280 | around 1.8 |
Features
- High yield of DNA extraction from various milk samples
- Including mastitic milk samples
- DNA sources from milk
- Bacteria (gram-negative; gram-positive)
- Yeasts
- Fungi
- Host cells
- Simple magnetic beads method
- No centrifuge needed
- No column needed
- No vacuum needed
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 50024S: 24 rxns
- Cat.# 50024L: 48 rxns
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