Magnetic Beads (DNA & RNA Purification)
Solid Phase Reversible Immobilization, SPRI beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are widely used for DNA purification because they are fast, simple and efficient. We have developed our own proprietary bead technology that offers distinct advantages over other SPRI beads.
Our Magnetic Beads (DNA & RNA Purification) combine BioDynami’s innovative chemistries with the reversible nucleic acid-binding properties of magnetic beads. These beads are uniquely engineered for effective purification by removing unwanted components such as salts, dNTPs, enzymes, primers, adapters, and other impurities. The beads are RNase-free, making them suitable for both DNA and more sensitive RNA applications—with no additional cost.
Our magnetic beads are optimized to selectively bind DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger, delivering performance comparable to other SPRI beads such as AMPure XP* and SPRIselect*. Purified DNA and RNA are free of contaminants and impurities, making them ideal for downstream applications requiring high-quality nucleic acids. The beads are versatile and can be used for NGS library purification, PCR fragment cleanup, molecular cloning, or nucleic acid concentration.
The beads can also be used for size selection of DNA fragments ranging from 150 bp to 800 bp by adjusting the bead-to-sample volume ratio and performing single or double-size selection. Furthermore, the beads are an ideal choice for NGS library preparation and can be easily integrated into standard workflows, as the volume ratios are consistent with protocols using conventional magnetic beads.
Features
- Effective recovery of DNA and RNA samples
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- DNA fragments >100 base pairs
- RNA fragments >200 bases
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- Elution in small volume without losing yield
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- As low as 10 ul is OK
- Ideal for precious, limited amount, and low-concentration samples
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- Cost effective alternative to other beads such as AMPure XP and SPRIselect with equivalent performance
Magnetic beads recovery rate. dsDNA and ssDNA of genomic DNA, 1 kb DNA, and 200 bp DNA fragments were used. Total RNA was also tested.
Comparison of elution volume vs yield. 40 ul, 20 ul, 15 ul, and 10 ul of elution volumes were used. BioDynami magnetic beads have better recovery rates at low elution volume when compared to other SPRI beads such as AMPure XP. Genomic DNA and NGS library samples are used for comparison.
Applications
- NGS Library preparation
- Purification of PCR fragments
- Purification of DNA and RNA fragments
- Molecular Cloning
- Other applications requiring purified DNA and RNA
Protocol & MSDS Download
Product Category
Cat.# & Volume
- Cat.# 40051S: 5 ml
- Cat.# 40051M: 20 ml
- Cat.# 40051L: 100 ml
Related Products
- Magnetic Beads (microRNA & Oligo Purification)
- Magnetic Beads (Short Oligo Purification)
- Magnetic Beads (PCR Purification)
- Magnetic Beads (tRNA Purification)
- DNA Concentrator (Magnetic Beads)
- cfDNA Purification Kit (Magnetic Beads)
- miRNA Purification Kit (tRNA Depletion)
- Plasmid Purification Magnetic Beads (RNA Depletion)
- RNA Contamination Removal Magnetic Beads (gDNA Purification)
- RNase-free RNA Removal (Magnetic Beads)
Background info
- Functionalized Magnetic Nanoparticles for Nucleic Acid Purification
- Magnetic particles for the separation and purification of nucleic acids
- Magnetic Beads Based Nucleic Acid Purification
* AMPure XP and SPRIselect are trade marks of Beckman Coulter.