Awaiting product image

Lysozyme (20 mg/ml)

10 kb DNA Ladder

$42 – $168

Clear
Categories: ,

Description

Lysozyme (20 mg/ml)

Lysozyme is an enzyme of 14.3 kDa (129 amino acids) with an isoelectric point of 10.7, existing in a wide variety of organisms and playing an important immunity role. Lysozymes can be classified into three types: c-type (chicken type), g-type (goose type), and i-type (invertebrate type).

Lysozyme is a glycosidase that can hydrolyze the β(1–4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine in the membranes of peptidoglycan, especially in Gram-positive bacteria. The peptidoglycan forms a layer outside to protect bacteria and also keep the bacteria shape. Gram-positive cells are very sensitive to lysozyme because the cell walls contain a high proportion of peptidoglycan. Gram-negative bacteria are less sensitive because of the lower proportion of peptidoglycan.

Lysozyme is characterized by the ability to break down the bacterial cell wall, improve genomic DNA and plasmid extraction, protein extraction, and inclusion body purification. Lysozyme from chicken egg whites has a higher heat tolerance than other sources, making it more effective for the lysis of gram-positive bacteria. It also improves the lysis of gram-negative bacteria.

Lysozyme is often used with RNase A for genomic DNA and plasmid extraction and is active over a broad range of pH values (6.0 to 9.0).

Features

      • Ready-to-use solution format
      • Optimal activity in a pH range of 6.0-9.0.

–Applications

      • Genomic DNA and plasmid extraction
      • Protein purification from inclusion bodies
      • Chitin hydrolysis
      • Degradation of bacterial cell wall

 

–

–

Related Products

 

Contents

Lysozyme (20 mg/ml)

Cat.# 40111S: 1 ml (20 mg/ml)
Cat.# 40111L: 5 ml (20 mg/ml)

Protocol & MSDS

Lysozyme (20 mg/ml)  (Cat.# 40111S; 40111L)

Protocol download
MSDS download

Related products