PCR, RT-PCR, qPCR
Amplification Reagents: PCR, RT-PCR, qPCR
BioDynami amplification reagents are available as 2X master mix or one-step reagents for a variety of applications, such as PCR, RT-PCR, and qPCR.
Amplification Supporting Reagents
BioDynami support reagents for enhancement of quality and specificity of amplification.
2X PCR Master Mix
2X PCR Master Mix is a ready-to-use 2X master mix solution containing Taq DNA polymerase, dNTPs, MgCl2 and stabilizers. The master mix allows the setup of the reactions in less than a minute by just adding primers and DNA templates, saves time and reduces contamination due to the fewer pipetting steps required for reaction set up, particularly when preparing large numbers of reactions. The mix is optimized for efficient and robust amplification of DNA templates with reproducibility and high yields.
2X qPCR Master Mix (Dye-Based and Probe-Based)
2X qPCR Master Mix is a ready-to-use 2X master mix solution optimized for real-time qPCR detection and quantification of target DNA with minimal or no optimization required. The master mix includes Taq DNA polymerase, dNTPs, MgCl2 and stabilizers. The master mix makes it easy for reaction setup by adding DNA templates and primers/probes, saves time and reduces contamination with fewer pipetting steps.
One-Step RT-PCR Kit
One-Step RT-PCR Kit is a convenient and quick solution for performing RT-PCR in a single step. RT-PCR is a well-used technique to amplify double-stranded DNA fragments from RNA templates. The One-Step RT-PCR Kit combines reverse transcription and PCR into one reaction step, which eliminates the need for two-step reactions. The one-step strategy avoids the transfer of reaction mixtures from the reverse transcription step to the PCR step, and reduces the contamination risk and minimizes tedious sample handling.
One-Step RT-qPCR Kit (Dye-Based and Probe-Based)
One-Step RT-qPCR Kit is a fast and convenient solution for performing real-time PCR. The kit combines reverse transcription and QPCR into one reaction, which eliminates the need for two step reactions with the additional transfer samples between the reverse transcription and QPCR steps, thus minimizing sample handling and reducing the risk of contamination.
Amplification Supporting Reagents
BioDynami support reagents for enhancement of quality and specificity of amplification.