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Milk DNA Extraction Kit (Magnetic Beads)

RNase A (Molecular Biology Grade, DNase-free), 10 mg/ml

$108 – $167

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Description

Milk DNA Extraction Kit (Magnetic Beads)

 

The Milk DNA Extraction Kit (Magnetic Beads) was developed for the extraction of nucleic acids from various sources in milk, including bacteria (both gram-negative and gram-positive), fungi, yeast, and host cells. It is also suitable for extracting DNA from mastitic milk samples. This magnetic bead-based kit offers a simple and reliable method for DNA extraction from milk samples, eliminating the need for tedious centrifugation steps.

The process begins with enzymatic digestion and lysis buffer, which breaks down bacteria, yeast, fungi, and host cells. Magnetic beads are then added to bind the DNA released from the lysed cells. After the initial DNA separation from the beads, a second bead-binding step is performed to remove additional impurities. Finally, the DNA is washed with ethanol and can be eluted in either elution buffer or TE buffer.

The standard input volume for milk is 500 µl. The yield of milk DNA extraction may vary depending on several factors, such as the bacterial species, type of milk, and cell density. The extracted DNA is suitable for a wide range of downstream applications, including PCR, qPCR, hybridization, restriction digestion, DNA sequencing, and Next-Generation Sequencing (NGS).

Milk DNA Extraction -BioDynami data

DNA from milk samples were isolated and loaded on a 1% agarose gel. DNA ladder: BioDynami 1 kb DNA Ladder (Cat.# 10004L).

Milk DNA Extraction -BioDynami-Specification

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Features of Milk DNA Extraction Kit

  • High yield of DNA extraction from various milk samples
    • Including mastitic milk samples
  • DNA sources from milk
    • Bacteria (gram-negative; gram-positive)
    • Yeasts
    • Fungi
    • Host cells
  • Simple magnetic beads method
    • No centrifuge needed
    • No column needed
    • No vacuum needed

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Contents

Milk DNA Extraction Kit (Magnetic Beads) (Cat.# 50024S)

Milk Lysis Buffer
MLQ Buffer
Pre-ISG Buffer
Milk A Beads
Milk B Beads
Lysozyme
Proteinase K
Elution Buffer

Milk DNA Extraction Kit (Magnetic Beads) (Cat.# 50024L)

Milk Lysis Buffer
MLQ Buffer
Pre-ISG Buffer
Milk A Beads
Milk B Beads
Lysozyme
Proteinase K
Elution Buffer

Protocol & MSDS

Milk  DNA Extraction Kit (Magnetic Beads) (Cat.# 50024S and 50024L)

Protocol Downoload
MSDS download

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