Description

DNA Normalization Magnetic Beads (NGS, PCR, gDNA)

The DNA Normalization Magnetic Beads (NGS, PCR, gDNA) was developed for normalization of NGS libraries, PCR fragments, sheared genomic DNA, and genomic DNA based on our magnetic bead technology. Magnetic beads has DNA-binding capacity. With the limitation of the DNA-binding capacity, normalized amount of DNA is recovered, and excess unbound DNA is separated.

The normalized DNA concentration can be obtained from DNA samples of various concentrations. Traditional fluorescent methods of DNA quantification require the setup of a standard curve from known DNA concentrations, compare with DNA samples from various sources, which would need extensive efforts and operating reagents. The protocol is simple, fast, centrifugation free, and filtration free. Additional DNA quantification and dilution is not necessary after DNA normalization with magnetic beads. Labor, time, and reagent are saved with the normalization beads.

The kit (Cat.# 40062) also includes unbound DNA recovery beads, which can be used for the recovery of unbound excess DNA during DNA normalization.

 

Normalization of NGS libraries. The input NGS library amount and normalized NGS library amount are indicated.

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Normalization of PCR fragments. The input PCR amount and normalized PCR amount are indicated.

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Normalization of genomic DNA samples. The input genomic DNA amount and normalized genomic DNA amount are indicated.

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Specifications

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Features

• Consistent normalization of various sources: NGS libraries, PCR fragments, and genomic DNA
• Recovery of excess unbound DNA (Cat.# 40062S and 40062L)
• Excellent dynamic range
  • • NGS library, PCR, sheared genomic DNA: 7-fold difference in input DNA
    • Genomic DNA: 10-fold difference in input DNA
• Simple and fast protocol with double-stranded DNA output
• No fluorescent quantification needed
• No centrifugation, no filtration

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Applications

• NGS library normalization
• Targeted enrichment
• PCR normalization
• Genomic DNA and sheared genomic DNA normalization

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