Proteinase K (recombinant, PCR grade)

Proteinase K is a serine protease that exhibits broad protein cleavage activity. It is well-used for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid.

Proteinase K has higher proteolytic activity in the presence of SDS and urea. The recombinant proteinase K has high activity and works in a wide range of pH and temperature. The recommended Proteinase K working concentration is 50–100 μg/ml.

Features

• • • Ready-to-use solution format
    • Optimal activity between 20 and 60°C. High activity between 50 to 55°C.
    • Optimal activity in a pH range of 4.5-12.0; High activity in the pH range of 7.5-11.5.
    • No detectable DNase, RNase, endonuclease, and exonuclease activities.

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Applications

• • • Inactivation of DNases, RNases and other enzymes in reactions
    • Removal of DNases and RNases for extraction of genomic DNA and RNA from tissues or cells
    • Plasmid DNA extraction
    • Enhancement of PCR fragment cloning efficiency
    • PCR purification

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