Sticky End Ligation Master Mix
The Sticky End Ligation Master Mix is developed for DNA fragment ligation of sticky end with 2-4 bases of overhangs in just 2-4 minutes. The 2X ligation master mix contains T4 DNA Ligase and proprietary components for ligation reaction. The mixed components improve the cohesive end ligation efficiency significantly with the substrates of typical 2-4 bases of overhangs.
The Sticky End Ligation Master Mix can be used for the ligation of the following types of DNA fragments:
The protocol for the Sticky End Ligation Master Mix is very simple: just mix the DNA fragments with the 2X Master Mix. The ligation of DNA fragments with sticky ends is fast at room temperature with high efficiency. Our modified downstream transformation protocol can save efforts and time for users.
Ligations with sticky-end DNA fragments using the Sticky End Ligation Master Mix between 1-10 minutes at room temperature. The ligated plasmids were transformed into DH-5α competent cells and grown on plates with LB medium at 37°C overnight.
Features
- Quick ligation in just 2-4 minutes for sticky ends (cohesive ends)
- Room temperature (20-25°C) ligation
- 2X Master Mix format made it easy to set up reaction
- Small ligation volume compatible with low DNA amount
- Modified transformation protocol with fewer steps and less time
Protocol & MSDS Download
Kit Components
- Cat.# 40103S: 50 rxns
- Cat.# 40103L: 250 rxns