Description
Rapid Ligation Master Mix
The Rapid Ligation Master Mix is optimized for DNA fragment ligation of sticky end or blunt end in only 5 minutes. The ligation reagent is a 2X master mix containing T4 DNA Ligase and proprietary ligation enhancers. The components of the master mix improve the ligation efficiency significantly when using different types of double-stranded DNA fragments.
The Rapid Ligation Master Mix can ligate the following DNA fragments:
The Rapid Ligation Master Mix makes it easy to set up ligation reactions by simply mixing the DNA fragments with the 2X Master Mix. The ligation of all types of DNA ends is fast at room temperature with high efficiency. The subsequent transformation protocol is also simplified to save both time and efforts.
Ligations with sticky ends or blunt ends using the Rapid Ligation Master Mix for different times at 20°C. The ligation plasmids were transformed into chemical competent cells DH-5α and grown on LB plates with carbenicillin at 37°C overnight.
Features
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- Fast ligation: only 5 minutes for sticky ends or blunt ends
- Room temperature (20-25°C) ligation
- Easy ligation reaction setup with the 2X Master Mix format
- Small volume of reaction made cloning possible with limited DNA input
- Simplified transformation protocol
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Related Products
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- DNA Fragmentation Enzyme Mix
- DNA Fragmentation & Blunting Enzyme Mix
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- Bacterial DNA Extraction Kit (Magnetic Beads)
- Blunt/TA Ligation Master Mix
- Sticky End Ligation Master Mix
