Description
dsDNA Quantification Kits: High Sensitivity (HS) and Broad Range (BR)
The dsDNA Quantification Kits can be used for double stranded DNA quantification with a cost saving of 65%. The DNA Quantification kits include Dye, Dilution Buffer, and two DNA Standards. Simply dilute the Dye with the Dilution Buffer, add DNA sample, then read the concentration using the Qubit® Fluorometer.
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- Cat.# 40042 dsDNA Quantification HS Kit, 100 assays and 500 assays
- Cat.# 40041 dsDNA Quantification BR Kit, 100 assays and 500 assays
dsDNA Quantification HS Kit
The dsDNA Quantification High Sensitivity Kit is developed for double stranded DNA quantification. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two DNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 0.005 ng/µL to 120 ng/µL with a detection range of 0.1 ng – 120 ng, and is highly selective for double-stranded DNA over RNA.
The 1X working solution can be stored at 4°C and protected from light, stable for up to 5 days.
Features
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- Optimized condition for using with the Qubit® Fluorometer
- Uses the Qubit® dsDNA High Sensitivity assay setting
- Linear range: 0.1 ng – 120 ng dsDNA
- 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 5 days.
- Cuts costs by 65%
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DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.
Comparison of dsDNA HS kits. The performance of the BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.
dsDNA Quantification BR Kit
The dsDNA Quantification Broad Range Kit is developed for double stranded DNA quantification. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and two DNA Standards. Simply dilute the BR Dye with the BR Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit® Fluorometer. The assay is accurate for DNA concentrations from 0.2 ng/µL to 2000 ng/µL with a detection range of 4 ng – 2000 ng, and is highly selective for double-stranded DNA over RNA.
The 1X working solution can be stored at 4°C and protected from light, stable for up to 2 months.
Features
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- Kit was optimized for use with the Qubit® Fluorometer
- Uses the Qubit® dsDNA Broad Range assay setting
- Detection range is 4-2000 ng dsDNA
- 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 2 months.
- Great saving since the kit cuts the costs by 65%
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DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.
Comparison of dsDNA BR kits. The performance of the BioDynami dsDNA BR kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA BR kit.
dsDNA HS Kit and BR Kit cost comparison
Effect of contaminants in HS Kit and BR kit
Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.
Qubit is a registered trademark of ThermoFisher Scientific.
