Description
DNA Fragmentation Enzyme
The enzymes were developed for random fragmentation of genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) in a fast and simple way. The DNA fragments contain 5´-phosphates and 3´-hydroxyl groups.
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- Cat. # 40061 DNA Fragmentation Enzyme Mix
- Cat. # 40062 DNA Fragmentation & A-tailing Enzyme Mix
- Cat. # 40063 DNA Fragmentation & Blunting Enzyme Mix
Enzyme-based fragmentation of DNA is very simple and effective comparing to the mechanical DNA shearing methods. There are several advantages of enzymatic DNA fragmentation, include easy handling of many samples at the same time, and less loss of samples. Moreover, it does not require expensive capital expense for the equipment.
DNA Fragmentation Enzyme Workflow
The resulting DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS). Our Fragmentation Enzyme does not generate detectable sequencing bias. Sequence coverage is also consistent between enzymatic and mechanical fragmentation.
DNA fragment size is inversely correlated with the incubation time of step 1 at 20°C.
DNA Fragmentation Enzyme Mix (Cat. # 40061)
The enzyme mix was developed for enzymatic fragmentation of genomic DNA with random ends in a single step. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS).
DNA Fragmentation & A-tailing Enzyme Mix (Cat. # 40062)
The enzyme mix can be used for enzymatic fragmentation of genomic DNA and addition of A-tailing at 3’-ends of DNA in a single step. 3’-ends of DNA with A-tailing can be used in many downstream applications in the field of molecular biology such as next generation sequencing, PCR cloning, and TA-ligation etc.
DNA Fragmentation & Blunting Enzyme Mix (Cat. # 40063)
The enzyme mix was developed to generate random fragmentation of genomic DNA with blunt ends in a single step. The fragmented DNA can be used for applications such as Next-Generation Sequencing (NGS), blunt end ligation and PCR cloning.
Features
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- Fast enzymatic fragmentation: 30-45 minutes
- Simple Procedure: 3 minutes of hands-on time
- Works with both EDTA-free DNA and DNA resuspended in TE buffer
- Ideal for downstream reactions: NGS library prep, PCR cloning, TA-ligation, and blunt end ligation etc.
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Comparison of Fragmentation Enzymes
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