Description

Blunt/TA Ligation Master Mix

The Blunt/TA Ligation Master Mix is developed for ligation of difficult DNA fragments as a ready-to-use 2X master mix containing T4 DNA Ligase and ligation enhancers. The proprietary components of the master mix significantly improve ligation of difficult DNA fragments with either blunt-ends or single-base overhang ends.

The Blunt/TA Ligation Master Mix can ligate the following DNA fragments:

The Blunt/TA Ligation Master Mix makes it easy to set up ligation reactions by simply mixing the DNA fragments with the 2X Master Mix. The ligation of all types of DNA ends is fast at room temperature with high efficiency. The subsequent transformation protocol is also simplified to save both time and efforts.

Ligations with single-base overhangs using the Blunt/TA Ligation Master Mix with reactions for different times at 20°C (left) or at different temperatures for 15 minutes (right). Transformants resulting from triplicate plating indicate that the Master Mix works well at room temperature (20°C-25°C) in 15 minutes.

Comparison of T4 DNA ligase (high concentration), the Rapid Ligation Master Mix (Cat.# 40102) and Blunt/TA Ligation Master Mix (Cat.# 40101) for ligation of nick, sticky end, 3´ single-base overhang, and 5´ single-base overhang substrates. All ligations were incubated for 15 minutes except for the 5´ single-base overhang, which was incubated for 1 hour.

 

Features

• • • Easy ligation reaction set-up with the 2X Master Mix format
    • Small ligation volume enables cloning and subcloning with limited amount of DNA
      • • Save precious DNA samples
        • Possible to transform many strains of competent cells simultaneously
    • Simplified transformation protocol

–

Applications

• • • Blunt-end cloning
    • TA cloning
    • GC cloning
    • Cloning vector preparation
    • Library construction
    • Linker and adaptor ligation
    • Fragment assembly

 

–

–

Related Products

• • Rapid Ligation Master Mix
  • Sticky End Ligation Master Mix
  • DNA Fragmentation Enzyme Mix
  • DNA Fragmentation & Blunting Enzyme Mix
  • DNA Fragmentation & A-tailing Enzyme Mix
  • Plasmid MiniPrep High Throughput Kit (Magnetic Beads)
  • Plasmid MiniPrep Kit (Magnetic Beads)
  • Bacterial DNA Extraction Kit (Magnetic Beads)