Description
2X qPCR Master Mix
There are two types of master mix that can be selected:
• Dye-Based: Master mix with an intercalating fluorescent dye allows for DNA detection and analysis without using sequence-specific probes.
• Probe-Based: Master mix for DNA detection and analysis using sequence-specific probes.
2X qPCR Master Mix is a ready-to-use 2X master mix solution optimized for real-time qPCR detection and quantification of target DNA with minimal or no optimization required. The master mix includes Taq DNA polymerase, dNTPs, MgCl2 and stabilizers. The master mix makes it easy for reaction setup by adding DNA templates and primers/probes, saves time and reduces contamination with fewer pipetting steps.
Real-time qPCR can be divided into two categories based on the signal sources: probe-based and intercalating dye-based. The probe-based qPCR technologies are more specific as the expensive sequence-specific dual-labelled probe is used. One advantage of the probe-based qPCR is that multiple PCR fragments can be detected in the same reaction.
The low-cost intercalating dye-based technologies have several advantages, such as melting curve analysis after amplification and faster cycling than that probe-based qPCR. BioDynami’s 2X qPCR Master Mix uses a unique DNA intercalating dye to replace SYBR Green I, but with less PCR inhibition. The development increases the reliability and sensitivity of the Master Mix in reaction conditions.
2X qPCR Master Mix (Dye-Based) data: qPCR for human GAPDH over a 5-log range of input concentrations (20 ng – 2 pg cDNA) with triplicates at each concentration.
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2X qPCR Master Mix (Probe-Based) data: qPCR for human beta-actin over a 5-log range of input concentrations (50 ng – 5 pg cDNA) with triplicates at each concentration.
2X qPCR Master Mix provides specific, sensitive and reproducible quantification of cDNA, genomic DNA, viral DNA, and plasmid DNA with earlier quantification cycle values and broad-range detection. The Optimized buffer eliminates the non-specific amplification and primer dimers formation, and leads to high reaction specificity and sensitivity.
The Master Mix is compatible with most real-time thermal cyclers. An additional passive ROX dye is provided, and it is compatible across a variety of instrument platforms with the requirement of either a high or low reference signal. The Master Mix is an affordable and reliable reagent for real-time qPCR applications including high throughput assay, gene expression, DNA quantification, genotyping, and pathogen detection.
Features
- Specificity: Optimized buffer eliminates non-specific amplification and primer dimers formation
- Sensitivity: Detects low copy number target DNA
- Easy reaction setup: Convenient, ready-to-use 2X master mix format
- Minimal risk of contamination by reducing pipetting steps
- Fluorescent dye: Comparable to SYBR Green, with less PCR inhibition
–Applications
- High throughput qPCR
- Gene expression
- Genomic DNA quantification
- siRNA validation
- Genotyping
- Pathogen detection
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