DNA Fragmentation
BioDynami DNA fragmentation reagents are available for genomic DNA fragmentation. The enzymatic DNA fragmentation is fast and simple since DNA shearing equipment is not required.
DNA Ligation
BioDynami DNA Ligation Master Mix reagents are available for ligation of difficult types of DNA fragments with high efficiency.
NGS Enzyme
BioDynami NGS enzymes are available for NGS library preparation as stand-alone modules and also can be combined to work together. The enzymes are in a ready-to-use master mix format.
Proteinase K (20 mg/ml)
BioDynami Proteinase K are available for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid.
Lysozyme (20 mg/ml)
BioDynami Lysozyme (20 mg/ml) from chicken egg white for genomic DNA and plasmid extraction, protein purification, degradation of bacterial cell wall, chitin hydrolysis.
RNase A (Ribonuclease A), 10 mg/ml
BioDynami RNase A (10 mg/ml) from from bovine pancreas for genomic DNA and plasmid extraction, removal of non-hybridized regions of RNA/DNA hybrids, degradation of unwanted RNA in gene expression analysis, and RNase protection assays.
DNA Fragmentation
The enzymes were developed for genomic DNA fragmentation in a fast and simple way and are compatible with EDTA-free DNA and DNA resuspended in TE buffer.
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- DNA Fragmentation Enzyme Mix – Fast enzymatic DNA fragmentation in 30-45 min. Only 3 min of hands-on time. Ideal for NGS application.
- DNA Fragmentation & Blunting Enzyme Mix – Blunting enzymes were incorporated in addition of the above DNA fragmentation reagent. Ideal for NGS application, PCR cloning and blunt end ligation.
- DNA Fragmentation & A-tailing Enzyme Mix – A-tailing enzymes were incorporated in addition of the above DNA fragmentation reagent. Ideal for NGS application, PCR cloning and TA ligation
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Comparison of Enzymes for DNA fragmentation
DNA Ligation
BioDynami DNA Ligation Master Mix reagents are available for ligation of difficult types of DNA fragments with high efficiency.
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- Blunt/TA Ligation Master Mix – For ligation of difficult types of DNA fragments such as blunt ends and single overhang ends.
- Rapid Ligation Master Mix – For 5-minutes quick ligation of various types of DNA fragments such as sticky ends and blunt ends.
- Sticky End Ligation Master Mix – For quick ligation of DNA fragments with sticky ends.
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NGS Enzyme
BioDynami NGS enzymes are stand-alone modules and also can be combined to work together. The enzymes are in a ready-to-use master mix format.
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- End Repair/dA-tailing Master Mix – The Master Mix uses a one-step reaction combining DNA blunting, 5’ phosphorylation, and 3′ dA-tailing extension to convert randomly fragmented DNA for subsequent ligation with library adaptors.
- NGS Ligation Enzyme – For ligation of DNA fragments with NGS library adaptors.
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Proteinase K (20 mg/ml)
BioDynami Proteinase K Proteinase K is a serine protease that exhibits broad protein cleavage activity.
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- Proteinase K – The Proteinase K for protein removal, PCR purification, nucleic acid extraction such as genomic DNA, RNA, and plasmid..
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Lysozyme (20 mg/ml)
BioDynami Lysozyme (20 mg/ml) from chicken egg white for genomic DNA and plasmid extraction, protein purification, degradation of bacterial cell wall, chitin hydrolysis.
RNase A (Ribonuclease A), 10 mg/ml
BioDynami RNase A (10 mg/ml) from from bovine pancreas for genomic DNA and plasmid extraction, removal of non-hybridized regions of RNA/DNA hybrids, degradation of unwanted RNA in gene expression analysis, and RNase protection assays.