MHC Targeted Sequencing Kits
The kits were developed to capture the MHC locus from whole genome NGS libraries based on the CATCH-Seq technology. Moreover, kits combining NGS library prep and MHC locus capture are also available. The integration of library prep and MHC capture not only simplifies the procedure, but also enhances the capture efficiency. Comprehensive sequencing of the selected locus with our kit make it possible to detect SNPs, indels, and structural variants that are not covered by other MHC targeted sequencing reagents.
Our MHC kits enable the most cost-effective targeted sequencing of the human MHC locus, including all coding and non-coding regions.
Library multiplexing up to 96 samples is possible with the unique dual indexes (UDI). We have developed a UDI system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Kit Types
- MHC Capture Kit: MHC capture reagents only
- MHC Library Prep & Capture Kit: NGS library prep reagents and MHC capture reagents
Features
- Integration of library prep with capture, or capture alone
- Simplifies the procedure
- Enhances the capture efficiency
- Multiple choices of targeted MHC regions
- Full 3.8 MB of MHC region
- 2.1 MB Class I region
- 0.7 MB Class II region
- 1.1 MB Class III region
- 190 kb MHC core regions (22 MHC genes)
- Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond.
- Easy detection of SNPs, indels, and structural variants
- The only reagent provides intact sequence information
- Multiplexing: up to 96 samples can be pooled together
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 32011S/32011L: 24/96 rxns (MHC Capture)
- Cat.# 32012S/32012L: 24/96 rxns (MHC Capture & Library Prep)
- Cat.# 32013S/32013L: 24/96 rxns (MHC Class I Capture)
- Cat.# 32014S/32014L: 24/96 rxns (MHC Class I Capture & Library Prep)
- Cat.# 32015S/32015L: 24/96 rxns (MHC Class II Capture)
- Cat.# 32016S/32016L: 24/96 rxns (MHC Class II Capture & Library Prep)
- Cat.# 32017S/32017L: 24/96 rxns (MHC Class III Capture)
- Cat.# 32018S/32018L: 24/96 rxns (MHC Class III Capture & Library Prep)
- Cat.# 32021S/32021L: 24/96 rxns (MHC Core Capture)
- Cat.# 32022S/32022L: 24/96 rxns (MHC Core Capture & Library Prep)
Related Products
- NGS DNA Library Prep Kit
- PCR-free NGS DNA Library Prep Kit
- NGS Low Input DNA Library Prep Kit
- ChIP-Seq Library Prep Kit
- NGS Cell-free DNA Library Prep Kit
- NGS FFPE DNA Library Prep Kit
- Bisulfite Sequencing Library Prep Kit
- Methylation Specific Bisulfite-Seq Library Prep Kit
- NGS Single Stranded DNA Library Prep Kit
- NGS Ancient DNA Library Prep Kit
- NGS DNA Fragmentation & Library Prep Kit
- RNA Seq Library Prep Kit
- Multiplexing Unique Dual Index Primers (Illumina Platform)
- NGS Library Quantification Standards With PCR Primers (Illumina platform)
- NGS Library Circularization Kit (MGI platform)
- Library Size Selection Kit (250-350 bp, Magnetic Beads)
- Library Size Selection Kit (300-450 bp, Magnetic Beads)
- Library Size Selection Kit (450-750 bp, Magnetic Beads)
- Library Size Selection Kit (>5 kb, Magnetic Beads)
- Library Size Selection Kit (>10 kb, Magnetic Beads)
- DNA Normalization Magnetic Beads (NGS, PCR, gDNA)