Genomic DNA Extraction Kit (HMW, Magnetic Beads)
- Cat.# 50014: gDNA extraction kit for cells
- Cat.# 50015:Â gDNA extraction kit for blood
- Cat.# 50016:Â gDNA extraction kit for tissues
The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.
The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.
A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.
Cultured Cell samples
Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Blood samples
Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Tissue samples
Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Specifications
Cell Samples | Blood Samples | Tissue Samples | |
Technology | Magnetic beads | Magnetic beads | Magnetic beads |
Sample amount | Up to 0.5 million cells | Up to 200 µl whole blood | Up to 10 mg tissue |
Genomic DNA size | 50-150 kb (tube tapping); 40-100 kb (tube vortexing) | 50-150 kb (tube tapping); 40-100 kb (tube vortexing) | 50-150 kb (tube tapping); 40-100 kb (tube vortexing) |
Genomic DNA yield | 3-6 µg (0.25 -0.5 million cells) | 4-12 µg (200 µl blood) | Up to 30 µg (dependent on the tissue) |
Elution volume | 100 µl | 100 µl | 100 µl |
O.D. 260/280 | 1.8-2.0 | 1.8-2.0 | 1.8-2.0 |
O.D. 260/230 | Above 2.0 | Above 2.0 | Above 2.0 |
Features
- High molecular weight DNA: 50 kb to 150 kb
- High purity
- Simple magnetic beads method
- No centrifuge needed
- No column needed
- No vacuum needed
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 50014S/50014L (for cells): 50/200 rxns
- Cat.# 50015S/50015L (for blood): 50/200 rxns
- Cat.# 50016S/50016L (for tissues): 50/200 rxns
- Cat.# 50016SP/50016LP (for tissues): 50/200 rxns (with Pestle Set)