ssDNA Quantification Kit
- Cat.# 40043, 200-µL Assay: standard 0.5 ml assay tubes needed
- Cat.# 40213, 100-µL Assay (half-volume): Assay tube adaptor and 100-µL assay tubes required
The ssDNA Quantification Kit is developed for single stranded DNA quantification. The kit includes ssDNA Dye, ssDNA Dilution Buffer, and two ssDNA Standards. Simply dilute the ssDNA Dye with the ssDNA Dilution Buffer, add DNA sample (volume from 1-20 μL), then read the concentration using the Qubit Fluorometer. The assay is accurate for DNA concentrations from 50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
A series of input ssDNA (200, 400, 600, 800, 1000, and 1200 ng) was used.
Our kit detects ssDNA by using fluorescent dye that enables sensitive single stranded DNA quantification , including ssDNA viruses, synthetic ssDNA, first-strand cDNA synthesis, denatured DNA, and bisulfate-converted DNA etc. ssDNA quantification is essential for the study of the biological process involves ssDNA.
Comparison of BioDynami ssDNA Kit with Thermo Fisher kit.
We have developed the Qubit assays with the volume of 100 µL. The volume is half of the standard 200 µL assay, and the reagent costs are reduced to half of the 200 µL assay. The ssDNA kits can save 67% (200 µL assay) and 83% (half-volume 100 µL assay) of the costs as compared to ThermoFisher’s Qubit ssDNA kits.
Cost comparison of ssDNA Kits.
Contaminant | Concentration in sample | % of signal change |
Sodium chloride | 100 mM | 1.5% |
Calcium chloride | 1 mM | 3.3% |
Magnesium chloride | 5 mM | 1.2% |
Phenol | 0.2% | 1.0% |
Chloroform | 2% | 0.3% |
Ethanol | 10% | 1.5% |
SDS | 0.01% | 1.6% |
Triton X-100 | 0.1% | -1.4% |
Polyethylene glycerol | 1% | 2.3% |
ATP | 0.1 mM | 0.7% |
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay.
Features
- Optimized for use with the Qubit Fluorometer
- Uses the Qubit ssDNA assay setting
- Linear range: 1-200 ng ssDNA
- Cost saving
- Save 62% (200-µL Assay)
- Save 79% (100-µL Assay)
Additional Features for 100-µL assays
- Reduced assay volume to 100 µL
- Cut the costs to 50% as compared to standard 200-µL assays
- High-throughput compatible: The 96-well plate racks and multichannel pipettes can be used.
- Shorten assay preparation time by 8-12 folds.
Protocol & MSDS Download
- Cat.# 40043S; 40043L
- Cat.# 40213S; 40213L
Kit Components
- ssDNA Dilution Buffer
- ssDNA Dye
- ssDNA Standard 1
- ssDNA Standard 2
Related Products
- dsDNA Quantification Broad Range Kit (for Qubit fluorometer)
- dsDNA Quantification High Sensitivity Kit(for Qubit fluorometer)Â
- Short Oligo Quantification Kit (for Qubit fluorometer)
- RNA Quantification High Sensitivity Kit (for Qubit fluorometer)
- Assay Tubes (Qubit Fluorometer)
- Assay Tube Adaptor (For Qubit Fluorometer, Half-Volume 100 µL Assay)
- dsDNA Quantification Broad Range Kit (for microplate reader)
- dsDNA Quantification High Sensitivity Kit (for microplate reader)
Note: Qubit is a registered trademark of ThermoFisher Scientific.