Description
End Repair/dA-Tailing Master Mix
The End Repair/dA-Tailing Master Mix is developed for converting 500 pg – 1 μg of fragmented DNA to repaired DNA with 5′ phosphorylated, 3′ dA-tailed ends. The Master Mix uses a one-step reaction combining DNA blunting, 5’ phosphorylation, and 3′ dA-tailing extension to convert randomly fragmented DNA for subsequent ligation with library adaptors. The Master Mix can be coupled with the downstream NGS Ligation Master Mix (Cat # 40105) to enables preparation of high-quality NGS libraries.
The End Repair/dA-Tailing Master Mix can work on the following DNA fragments:
The master mix format and the proprietary chemistry makes reaction setup easy and workflow simple. This is an affordable choice for both large and small projects to pursue fast time and high quality, as the Master Mix provides a flexible solution for NGS library prep for different types of DNA input. The Master Mix ensures both sensitivity and efficiency for DNA samples by maximizing the yield for the subsequent ligation process of the sequencing library preparation.
Features
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- Combines the following reactions into one single step
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- DNA blunting
- 5’ phosphorylation
- 3’ dA-tailing extension
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- High efficiency for library conversion
- Limited GC bias across the whole genome
- Input DNA: 500 pg to 1 µg
- Combines the following reactions into one single step
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