Blunt/TA Ligation Master Mix

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Description

Blunt/TA Ligation Master Mix

The Blunt/TA Ligation Master Mix is developed for ligation of difficult DNA fragments as a ready-to-use 2X master mix containing T4 DNA Ligase and ligation enhancers. The proprietary components of the master mix significantly improve ligation of difficult DNA fragments with either blunt-ends or single-base overhang ends.

The Blunt/TA Ligation Master Mix can ligate the following DNA fragments:

DNA ligation

The Blunt/TA Ligation Master Mix makes it easy to set up ligation reactions by simply mixing the DNA fragments with the 2X Master Mix. The ligation of all types of DNA ends is fast at room temperature with high efficiency. The subsequent transformation protocol is also simplified to save both time and efforts.

blunt ta ligation efficiency

Ligations with single-base overhangs using the Blunt/TA Ligation Master Mix with reactions for different times at 20°C (left) or at different temperatures for 15 minutes (right). Transformants resulting from triplicate plating indicate that the Master Mix works well at room temperature (20°C-25°C) in 15 minutes.

ligation comparison

Comparison of T4 DNA ligase (high concentration), the Rapid Ligation Master Mix (Cat.# 40102) and Blunt/TA Ligation Master Mix (Cat.# 40101) for ligation of nick, sticky end, 3´ single-base overhang, and 5´ single-base overhang substrates. All ligations were incubated for 15 minutes except for the 5´ single-base overhang, which was incubated for 1 hour.

 

Features

      • Easy ligation reaction set-up with the 2X Master Mix format
      • Small ligation volume enables cloning and subcloning with limited amount of DNA
          • Save precious DNA samples
          • Possible to transform many strains of competent cells simultaneously
      • Simplified transformation protocol

Applications

      • Blunt-end cloning
      • TA cloning
      • GC cloning
      • Cloning vector preparation
      • Library construction
      • Linker and adaptor ligation
      • Fragment assembly

 

Related Products

 

Contents

Blunt/TA Ligation Master Mix

Cat.# 40101S: 50 reactions (250 ul)
Cat.# 40101L: 250 reactions (1250 ul)

Protocol & MSDS

Blunt/TA Ligation Master Mix (Cat.# 40101S; 40101L)

Protocol download
MSDS download