Description
NGS Ligation Enzyme
The NGS Ligation Enzyme was developed for preparation of high-quality libraries for next generation sequencing. The reagent was derived from the ligation step of our NGS library prep kits. The reagent contains Ligation Enzyme and Ligation Buffer with enhancers for high library conversion efficiency.
The proprietary components in the buffer improve ligation of NGS adaptors to dA-tailed DNA fragments dramatically. The ligation is performed at room temperature (20°C) for only 20 minutes with high efficiency. The enzyme is also optimized for use with the BioDynami End Repair/dA-Tailing Master Mix (Cat.# 40104S; 40104L).
Features
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- Fast: 20 minutes ligation at room temperature
- Simple reaction setup
- Higher library conversion efficiency
- Input DNA amount: From 1 ng to 1 ug
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