NGS DNA Library Prep Kit

The kit was developed for construction of high quality libraries for next generation sequencing (illumina and MGI Platforms). The kit needs double-strand DNA fragments (blunt and/or sticky) as input DNA for NGS library preparation, and is compatible with DNA fragments generated from both enzymatic methods and physical methods (sonication, nebulization etc.). Library multiplexing is possible with different types of indexes.

The kit was optimized for next generation sequencing (NGS) library preparation with different types of samples. Most DNA library preparation requires the ligation of sheared DNA fragments to library adaptors and the DNA library preparation is closely related to the quality of NGS data. With BioDynami’s unique DNA library preparation technologies, the fast and simple kit allows high-quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.

NGS Kit Workflow

Some genomic regions are very difficult to be covered evenly and usually result in a very low coverage rate or gap in these regions. Typical difficult regions are:

• With high GC content
• Have secondary structures: mainly due to repeat sequences
• The worst cases: have both high GC content and repeated sequences.

Example: the human TERT gene is one of the most difficult regions as shown above. NGS data showed that the BioDynami kit has the best performance in covering the extremely difficult human TERT gene region.

Comaprison of GC% vs coverage

Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Indexes are available for the MGI platform kits (Cat.# 34021).

Features

• Fast protocol
  • The hands-on time is only 10 minutes
  • The total protocol time is around 1.5 hours
• Less magnetic beads required for cleanup steps:
  • Save the cost more than 50%

Protocol & MSDS Download

• Download link

Cat.# & Rxns

• Cat.# 30023A/30023F/30023S/30023L: 24/48/96/192 rxns (illumina)
• Cat.# 34021S/34021L: 24/96 rxns (MGI)

Related Products

• PCR-free NGS DNA Library Prep Kit
• NGS Low Input DNA Library Prep Kit
• ChIP-Seq Library Prep Kit
• NGS Cell-free DNA Library Prep Kit
• NGS FFPE DNA Library Prep Kit
• Bisulfite Sequencing Library Prep Kit
• Methylation Specific Bisulfite-Seq Library Prep Kit
• NGS Single Stranded DNA Library Prep Kit
• NGS Ancient DNA Library Prep Kit
• NGS DNA Fragmentation & Library Prep Kit
• RNA Seq Library Prep Kit
• Multiplexing UDI Primers (Illumina Platform)
• Multiplexing UDl Primers & Adaptors (Illumina Platform)
• NGS Library Quantification Standards With PCR Primers (Illumina platform)
• NGS Library Circularization Kit (MGI platform)
• Library Size Selection Kit (250-350 bp, Magnetic Beads)
• Library Size Selection Kit (300-450 bp, Magnetic Beads)
• Library Size Selection Kit (450-750 bp, Magnetic Beads)
• Library Size Selection Kit (>5 kb, Magnetic Beads)
• Library Size Selection Kit (>10 kb, Magnetic Beads)
• DNA Normalization Magnetic Beads (NGS, PCR, gDNA)