Description
miRNA Purification Kit (tRNA Depletion)
The miRNA Purification Kit (tRNA Depletion) is developed for microRNA (miRNA) purification by removal of tRNA, 5S RNA, and other larger RNA contaminations.
microRNAs are typically around 22 nucleotides long and can be used in various downstream applications relating to gene expression regulation, miRNA profiling, and functional analysis. However, miRNA isolated from commercial microRNA extraction kits usually contain contaminations of tRNA, 5S RNA, and other larger RNA molecules. The contamination may result in decreased sensitivity or inconsistent data for applications such as PCR, RT-PCR, next-generation sequencing (NGS), microarray, etc.
We have developed a Solid Phase Reversible Immobilization (SPRI) beads-based technology to solve the problem. SPRI beads are well used for nucleic acid purification. The paramagnetic beads coated with carboxyl groups can reversibly bind to nucleic acid that are 100 base pairs or longer. However, with traditional SPRI beads, nucleic acids shorter than 100 bp are poorly recovered.
Our proprietary magnetic bead technology not only overcomes the short nucleic acid recovery hurdle, but also enables effective separation of miRNA from large RNA molecules. With this technology, the kit selectively purifies miRNA and removes tRNA, 5S RNA, and other larger RNA molecules. The magnetic beads can also remove impurities and unwanted components such as salts, proteins, dNTPs, and detergents.
Workflow of miRNA Purification kit
Features
- Purification of miRNA by removing tRNA, 5S RNA, and larger RNA contaminations
- Removal of unwanted components and other impurities
Examples of miRNA purification
Kit efficiency: miRNA recovery rate and tRNA depletion rate
Related Products
- Magnetic Beads (DNA & RNA Purification)
- Magnetic Beads (microRNA & Oligo Purification)
- Magnetic Beads (Short Oligo Purification)
- Magnetic Beads (PCR Purification)
- Magnetic Beads (tRNA Purification)
- DNA Concentrator (Magnetic Beads)
- cfDNA Purification Kit (Magnetic Beads)
- Plasmid Purification Magnetic Beads (RNA Depletion)
- RNA Contamination Removal Magnetic Beads (gDNA Purification)
- DNA Normalization Magnetic Beads (NGS, PCR, gDNA)
