Magnetic Beads (tRNA Purification)
Solid Phase Reversible Immobilization magnetic beads has been used for nucleic acids purification because they are effective, simple, and fast. The carboxyl groups coated beads are paramagnetic particles that reversibly bind to nucleic acid. However, there is a drawback for magnetic beads that it can only purify DNA/RNA that are 100 base pairs or longer. tRNA and other DNA/RNA fragments shorter than 100 base pairs are not effectively recovered. We have developed Magnetic Beads (tRNA Purification) to overcome the hurdle.
Workflow without large RNA/DNA contamination
Workflow with large RNA/DNA contamination
The Magnetic Beads (tRNA Purification) solves the tRNA and short DNA/RNA purification problem. The beads with our proprietary technology purify tRNA effectively by removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The magnetic bead reagents can be used for oligo (>70 nt) applications.
In the case of samples contaminated with RNA/DNA such as rRNA and DNA, our magnetic beads can effectively remove RNA/DNA that are 180 nt and larger. Purified tRNA are ideal for applications requiring high quality, as the fragments are free of impurities and contaminants.
Left panel: Comparison of tRNA and 70 nt oligos recovery. BioDynami beads (tRNA purification) successfully recovered DNA fragments both yeast tRNA and 70 nt oligos. Right panel: Depletion of 28S rRNA, 18S rRNA, and RNA of 180 nt and larger.
Recovery of tRNA and 70 nt oligos with BioDynami magnetic Beads (tRNA Purification). Yeast tRNA and 70 nt oligos were used as input. Input and recovered oligos were quantified with ssDNA Quantification kit (BioDynami Cat. # 40043) and RNA Quantification kit (BioDynami Cat. # 40044).
Features
- Purification of tRNA and oligos (>70 nt)
- tRNA
- RNA fragments 70 nt or longer
- DNA/RNA hybrid fragments 70 nt or longer
- Oligo and chimeric oligo 70 nt or longer
- dsDNA fragments 70 bp or longer
- ssDNA fragments 70 nt or longer
- Removal of larger RNA/DNA contamination:
- 18S rRNA
- 28S rRNA
- RNA/DNA> 180 nt
- Removal of unwanted components and other impurities
Protocol & MSDS Download
Cat.# & Volume
- Cat.# 40054S: 5 ml
- Cat.# 40054M: 20 ml
- Cat.# 40054L: 100 ml
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