Library Size Selection Kit (for Long-read Sequencing, Magnetic Beads)

The size selection ranges ideal for long-read sequencing:

• • Cat.# 20110: >5 kb
  • Cat.# 20111: >10 kb

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The Library Size Selection Kit (for Long-read Sequencing, Magnetic Beads) can be used for library size selection for long-read sequencing platforms (PacBio and Oxford Nanopore). Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

DNA size selection for long-read sequencing

Long-read sequencing, such as PacBio and Oxford Nanopore, uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions.  After the beads-binding step and rinsing step, the NGS library fragments are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

Features

• High specificity and high recovery of size selection
• 2 ranges for long-read sequencing size selection
  • >5 kb
  • >10 kb
• Rapid protocol: only 20 minutes
• Simple workflow
  • No columns required
  • No gel purification required
  • No centrifugation required
• Efficient removal of contaminants and unwanted components

Protocol & MSDS Download

• Download link

Cat.# & Rxns

• Cat.# 20110S/20110L (>5 kb): 24/96 rxns
• Cat.# 20111S/20111L (>10 kb): 24/96 rxns

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