dsDNA Quantification: High Sensitivity (HS) & Broad Range (BR)

The dsDNA Quantification Kits can be used for double-stranded DNA quantification with a cost saving of 67% (standard 200 µL assay) and 83% (half-volume 100 µL assay), respectively.

• 200-µL Assay: standard 0.5 ml assay tubes needed
  • Cat.# 40041S, 40041L: dsDNA Quantification BR Kit
  • Cat.# 40042S, 40042L: dsDNA Quantification HS Kit

• 100-µL Assay (half-volume): Assay tube adaptor and 150-µL assay tubes required
  • Cat.# 40211S, 40211L: dsDNA Quantification BR Kit (Half-Volume 100-ul Assay)
  • Cat.# 40212S, 40212L: dsDNA Quantification HS Kit (Half-Volume 100-ul Assay)

Features of 100-µL assays

• • Reduced assay volume to 100 µL
  • Cut the costs to 50% as compared to standard 200-µL assays
  • High-throughput compatible: The 96-well plate racks and multichannel pipettes can be used.
  • Shorten assay preparation time by 8-12 folds.

* Compared to ThermoFisher kits
** Assay tubes can be prepared in 96-well plate racks

 

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dsDNA Quantification HS Kit

The dsDNA Quantification High Sensitivity Kit is developed for double stranded DNA quantification. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two DNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for DNA concentrations from 0.005 ng/µL to 120 ng/µL with a detection range of 0.1 ng – 120 ng, and is highly selective for double-stranded DNA over RNA.

The 1X working solution can be stored at 4°C and protected from light, stable for up to 5 days.

Features

• Optimized condition for using with the Qubit Fluorometer
• Uses the Qubit dsDNA High Sensitivity assay setting
• Linear range: 0.1 ng – 120 ng dsDNA
• 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 5 days.
• Cuts costs by 67%

DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.

The BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.

Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.

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dsDNA Quantification BR Kit

The dsDNA Quantification Broad Range Kit is developed for double stranded DNA quantification. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and two DNA Standards. Simply dilute the BR Dye with the BR Dilution Buffer to make 1X working solution, add DNA sample, then read the concentration using the Qubit Fluorometer. The assay is accurate for DNA concentrations from 0.2 ng/µL to 2000 ng/µL with a detection range of 4 ng – 2000 ng, and is highly selective for double-stranded DNA over RNA.

The 1X working solution can be stored at 4°C and protected from light, stable for up to 2 months.

Features

• Kit was optimized for use with the Qubit Fluorometer
• Uses the Qubit dsDNA Broad Range assay setting
• Detection range is 4-2000 ng dsDNA
• 1X working solution (mixture of dye and dilution buffer) can be stored at 4°C and protected from light, stable for up to 2 months.
• Great saving since the kit cuts the costs by 67%

DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.

The BioDynami dsDNA BR kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA BR kit.

Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.

 

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Protocol & MSDS Download

• Download link

Cat.# & Assays

• Cat.# 40041S/40041L: 100/500 assays (BR)
• Cat.# 40211S/40211L: 200/1000 assays (BR 100-µl assay)
• Cat.# 40042S/40042L: 100/500 assays (HS)
• Cat.# 40212S/40212L: 200/1000 assays (HS 100-µl assay)

Related Products

• • ssDNA Quantification Kit (for Qubit fluorometer)
  • Short Oligo Quantification Kit (for Qubit fluorometer)
  • RNA Quantification High Sensitivity Kit (for Qubit fluorometer)
  • Assay Tubes (Qubit Fluorometer)
  • Assay Tube Adaptor (For Qubit Fluorometer, Half-Volume 100 µL Assay)
  • dsDNA Quantification Broad Range Kit (for microplate reader)
  • dsDNA Quantification High Sensitivity Kit (for microplate reader)

 

Note: Qubit is a registered trademark of ThermoFisher Scientific.