dsDNA Quantification Kit (for Microplate Reader)

Features

  • Double-stranded DNA quantification using microplate readers
  • Both Broad Range (BR) and High Sensitivity (HS) available
  • Cuts costs by 60%
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dsDNA Quantification Kit (for Microplate Reader)

The dsDNA Quantification Broad Range Kit and dsDNA Quantification High Sensitivity Kit are developed for double stranded DNA quantification using microplate readers. The DNA Quantification BR kit includes BR Dye, BR Dilution Buffer, and BR DNA Standards. The DNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and HS DNA Standards. Simply dilute the Dye with the Dilution Buffer, add DNA sample, then read the concentration using microplate reader. The BR assay is accurate in the linear range from 4 to 1000 ng and the HS assay is accurate in the linear range from 0.2 to 100 ng. Both kits are highly selective for double-stranded DNA over RNA.

The Quantification Kits have several advantages over traditional DNA quantitation such as sensitivity, stability, and tolerance of contaminants.

Both kits reduce the cost of DNA quantification by 60% as compared to other brands.

Features

  • Double-stranded DNA quantification using microplate readers
  • Both Broad Range (BR) and High Sensitivity (HS) available
  • Cuts costs by 60%

Selectivity and sensitivity of the kits

Comparison of BioDynami dsDNA Quantification Broad Range Kit with Thermo Fisher kit

Comparison of BioDynami dsDNA Quantification High Sensitivity Kit with Thermo Fisher kit.

Save 60% of the costs with BioDynami kits.

Common contaminants such as salts, free nucleotides, solvents, detergents, RNA, single stranded DNA, or protein are well tolerated in the assays.

Contaminant

Final concentration in the assay

Concentration in 10-μL sample

Result

Sodium chloride

10 mM

200 mM

OK

Magnesium chloride

2 mM

40 mM

OK

Sodium acetate

10 mM

200 mM

OK

Ethanol

1%

20%

OK

Phenol

0.1%

2%

OK

SDS

0.001%

0.02%

OK

Triton X-100

0.001%

0.02%

OK

dNTP

100 μM

2 mM

OK

BSA

20 μg/mL

400 μg/mL

OK

RNA

0.5X

0.5X

OK

ssDNA

0.5X

0.5X

OK

Tolerance assays: dsDNA Quantification Broad Range Kit

 

Contaminant

Final concentration in the assay

Concentration in 10-μL sample

Result

Sodium chloride

10 mM

200 mM

OK

Magnesium chloride

5 mM

100 mM

OK

Sodium acetate

10 mM

200 mM

OK

Ethanol

1%

20%

OK

Phenol

0.1%

2%

OK

SDS

0.01%

0.2%

OK

Triton X-100

0.003%

0.05%

OK

dNTP

100 μM

2 mM

OK

BSA

1 mg/mL

20 mg/mL

OK

RNA

0.5X

0.5X

OK

ssDNA

0.5X

0.5X

OK

Tolerance assays: dsDNA Quantification High Sensitivity Kit

 

Protocol & MSDS Download

Cat.# & Assays

  • Cat.# 40540B: 1000 assays (BR)
  • Cat.# 40540H: 1000 assays (HS)

Related Products

 

Note: Qubit is a registered trademark of ThermoFisher Scientific.

 

 

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