Qubit dsDNA Quantification Kits (100-µl Assay): HS and BR
The dsDNA Quantification Kits (100-µL assay) can be used for dsDNA quantification with a cost saving of 83% compared to ThermoFisher Qubit kits. The 100-µL assays can cut the assay costs to half as compared to the standard 200-µL assays. Assay tube adaptor and 150-µL assay tubes are required for the 100-µL assays.
Features of Qubit 100-µL assays
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- Reduce assay volume to 100 µL
- Cut the costs to 50% compared to standard 200-µL assays
- High-throughput: The 96-well plate racks and multichannel pipettes can be used.
- Assay preparation time: reduced by 8-12 folds.
Comparison: Standard 200-µL assay vs half-volume 100-ul Assay
Vendor | Cat.# | Kit | Assay volume | Assay tube type | Assay tube adaptor | Cost/assay | Cost percentage | High-through compatibility | ||
Reagent | Tube | Total | ||||||||
ThermoFisher | Q32853 | BR & HS kits | 200 µl | 500 µl | Not required | $0.81 | $0.26 | $1.07 | 100% | No** |
BioDynami | 40041L 40042L | BR & HS kits | 200 µl | 500 µl | Not required | $0.27 | $0.11 | $0.38 | 33%* | No** |
BioDynami | 40211L 40212L | BR & HS kits (100-µL assay) | 100 µl | 150 µl | Required | $0.14 | $0.07 | $0.21 | 17%* | Yes** |
* Compared to ThermoFisher kits
** Assay tubes can be prepared in 96-well plate racks
dsDNA Quantification HS Kit (100-µl Assay)
The dsDNA Quantification High Sensitivity Kit (100-µl Assay) is developed for double-stranded DNA quantification with the half-volume of 100-ul Assays. The 100-µL assays can cut the assay costs to half as compared to the standard 200-µL assays. The assay is accurate for DNA concentrations with a detection range of 0.1 ng – 120 ng, the same as the standard high sensitivity 200-µl assays.
Features
- Reduced assay volume to 100 µL
- High-throughput: The 96-well plate racks and multichannel pipettes can be used.
- Assay preparation time: reduced by 8-12 folds with the high-throughput setting.
- Cost saving by 50% compared to standard 200-µL assays
- Cost saving by 83% compared to ThermoFisher kits
DNA selectivity and sensitivity of the HS kit. A series of input DNA (20, 40, 60, 80, 100, and 120 ng) was used with or without the contamination of RNA.
The BioDynami dsDNA HS kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA HS kit.
dsDNA Quantification BR Kit (100-µl Assay)
The dsDNA Quantification Broad Range Kit (100-µl Assay) is developed for dsDNA quantification with the half-volume of 100-ul Assays. The 100-µL assays can cut the assay costs to half as compared to the standard 200-µL assays. The assay is accurate for DNA concentrations with a detection range of 4 ng – 2000 ng, the same as the standard broad range 200-µl assays.
Features
- Reduced assay volume to 100 µL
- High-throughput: The 96-well plate racks and multichannel pipettes can be used.
- Assay preparation time: reduced by 8-12 folds with the high-throughput setting.
- Cost saving by 50% compared to standard 200-µL assays
- Cost saving by 83% compared to ThermoFisher kits
DNA selectivity and sensitivity. A series of input DNA (200, 400, 600, 800, and 1000 ng) was used with or without RNA contamination.
The BioDynami dsDNA BR kit is nearly identical to that of Thermo Fisher’s Qubit dsDNA BR kit.
Protocol & MSDS Download
Product Category
Cat.# & Assays
- Cat.# 40211S/40211L: 200/1000 assays (BR 100-µl assay)
- Cat.# 40212S/40212L: 200/1000 assays (HS 100-µl assay)
Related Products
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- dsDNA Quantification Broad Range Kit (for Qubit fluorometer)
- dsDNA Quantification High Sensitivity Kit (for Qubit fluorometer)
- ssDNA Quantification Kit (for Qubit fluorometer)
- Short Oligo Quantification Kit (for Qubit fluorometer)
- RNA Quantification High Sensitivity Kit (for Qubit fluorometer)
- Assay Tubes (Qubit Fluorometer)
- Assay Tube Adaptor (For Qubit Fluorometer, Half-Volume 100 µL Assay)
- dsDNA Quantification Broad Range Kit (for microplate reader)
- dsDNA Quantification High Sensitivity Kit (for microplate reader)
Note: Qubit is a registered trademark of ThermoFisher Scientific.
Background info