DNA Removal Magnetic Beads (for RNA Purification)
The DNA Removal Magnetic Beads (for RNA Purification) is developed for the efficient elimination of genomic DNA contamination in RNA samples. During RNA extraction, genomic DNA is frequently co-purified due to nuclear lysis. This contamination poses a significant challenge for downstream applications, particularly those involving polymerase chain reaction (PCR). The issue is especially critical when quantifying low-copy transcripts or processing low-input samples, where residual DNA can lead to inaccurate quantification and false results.
We have developed a unique beads-based system that completely removes DNA contamination using Solid Phase Reversible Immobilization (SPRI) technology. The reagent combines RNase-free DNase with magnetic beads—paramagnetic particles coated with carboxyl groups that reversibly bind nucleic acids. Our proprietary magnetic beads can be stored at -20°C to maintain maximal DNase activity.
The reagent is formulated with RNase-free DNase, ensuring the complete degradation of DNA while preserving RNA integrity. The DNase and all other reagent components are thoroughly removed during the subsequent ethanol wash steps. The magnetic bead-based protocol offers a simple and fast workflow, eliminating the need for tedious, repetitive centrifugation steps required by traditional column-based methods.
Left panel: DNA removal from an RNA sample with gDNA contamination. Right panel: DNA removal and RNA recovery comparison using different samples: total RNA, mRNA and genomic DNA. Input and recovery rates are indicated.
This system effectively removes DNA while simultaneously recovering intact RNA. In addition to DNA, unwanted contaminants such as enzymes, proteins, salts, and other impurities are also eliminated during the process. The purified RNA is ready for use in a wide range of sensitive downstream applications, including enzymatic treatments, hybridization, cDNA synthesis, RT-PCR, RT-qPCR, and NGS library preparation.
Features
- Effective removal of DNA by RNase-free DNase
- High RNA recovery rate via magnetic beads
- Simultaneous DNA removal and RNA recovery in a single step
- Efficient elimination of unwanted components and impurities
- Simple and fast beads-based protocol
Protocol & MSDS Download
Product Category
Cat.# & Volume
- Cat.# 47063S: 2.5 ml
- Cat.# 47063L: 10 ml
Related Products
- Magnetic Beads (DNA & RNA Purification)
- Magnetic Beads (microRNA & Oligo Purification)
- Magnetic Beads (Short Oligo Purification)
- Magnetic Beads (PCR Purification)
- Magnetic Beads (tRNA Purification)
- DNA Concentrator (Magnetic Beads)
- cfDNA Purification Kit (Magnetic Beads)
- miRNA Purification Kit (tRNA Depletion)
- Plasmid Purification Magnetic Beads (RNA Depletion)
- RNA Contamination Removal Magnetic Beads (gDNA Purification)
- RNase-free RNA Removal (Magnetic Beads)
Background info