Breast Cancer Panel Targeted Sequencing Kits

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Description

Breast Cancer Panel Targeted Sequencing Kits

 

Breast Cancer Panel Library Prep & Capture Kit (illumina)

The kits were developed for breast cancer genetic testing based on the CATCH-Seq technology. Moreover, kits combining NGS library prep and capture are also available. The integration of library prep and capture simplifies the procedure, and enhances the capture efficiency. Comprehensive sequencing of the 117 breast cancer genes make it possible to detect indels, SNPs, and structural variants that are not covered by other targeted sequencing reagents.

Breast Cancer Panel Capture Kit: Capture reagents only. Library Prep reagents are not included.

Breast Cancer Panel Library Prep & Capture Kit: Combination of library prep reagents and capture reagents.

ABCB1 BRCA2 DYNLRB1 FOXA1 IGFBP1 MAPK3 N4BP2L1 PIK3CA SEC16A TP53
ABCG2 CCND1 EGF FUS IGFBP3 MAPK8 NBR2 PIK3R1 SERPINE1 TRIM46
AKT1 CCND2 EGFR GATA3 IL6 MDM2 NEK2 PLAU SFN TUSC2
APC CDH1 EP300 GDPD3 INPP5E MGMT NME1 PNMT SFRP1 TWIST1
AR CDK2 ERBB2 GLI1 ITCH MINE1 NME2 PPP1R1B SNAI2 VEGFA
ARHGAP9 CDKN1A ERBB2 GPR137 JUN MKI67 NOTCH1 PTEN SRC WEE1
ATM CDKN1C ERBB3 GRB7 KLLN MLH1 NPRL2 PTGS2 STARD3 XBP1
BAD CDKN2A ESR1 GSTP1 KRT5 MMP2 NR3C1 PYCARD TCAP ZAR1L
BAP1 CSF1 ESR2 HIC1 LPAR6 MMP9 OVCA2 RASSF1 TFF3 ZMYND10
BCL2 CTNNB1 FBXO32 ID1 MAP1LC3A MUC1 PA2G4 RB1 TGFB1
BIRC5 CTSD FGFR1 IGF1 MAP3K1 MUC16 PGAP3 RET THBS1
BRCA1 CYB561D2 FGFR2 IGF1R MAPK1 MYC PGR RPL41 THBS3

Our breast cancer panel kits enable the most cost-effective targeted sequencing of the 117 breast cancer genes, including all coding and non-coding regions.

 

Two index types are available:

Single index: For kits with 24 reactions. Each of the index primers (#1~#24) has a unique 6-base index sequence that can be used to identify libraries. Details about the index can be found here.

Unique dual index: For kits with 96 and 384 reactions. Multiplexing up to 96 libraries is possible. Details about the unique dual index can be found here.

 

Features:

    • Two options: combination of library prep with capture, or capture alone
        • Simplifies the procedure
        • Enhances the capture efficiency
    • Full genomic regions of 117 genes
        • Covers ~9 MB regions
        • Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond.
    • Easy detection of SNPs, indels, and structural variants
        • The only reagent provides intact sequence information
    • Low cost
    • Sample multiplexing:  Further reduces the cost

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Contents

Breast Cancer Panel Capture Kit (Cat.# 32025)

Probe
RS Buffer
W1 Buffer
W2 Buffer
Non-index Primers
Index Primers
PCR mix

Breast Cancer Panel Library Prep & Capture Kit (Cat.# 32026)

DL1 Buffer
DL1 Enzyme
DL2 Buffer
DL2 Enzyme
Probe
RS Buffer
W1 Buffer
W2 Buffer
Non-index Primers
Index Primers
PCR mix

Protocol & MSDS

Breast Cancer Panel Capture Kit (Cat.# 32025)

Protocol download
MSDS download

Breast Cancer Panel Library Prep & Capture Kit (Cat.# 32026)

Protocol download
MSDS download