DNA Normalization Magnetic Beads (50-300 ng Input)
The DNA Normalization Magnetic Beads (50-300 ng Input) was developed for normalization of NGS libraries, PCR fragments, and sheared genomic DNA based on our magnetic bead technology. Magnetic beads has DNA-binding capacity. With the limitation of the DNA-binding capacity, normalized amount of DNA is recovered, and excess unbound DNA is separated.
 Traditional methods of DNA normalization require the setup of a standard curve, compare with DNA samples from various sources, which would need extensive efforts and operating reagents. The protocol is simple, fast, centrifugation free, and filtration free. Additional DNA quantification and dilution is not necessary after DNA normalization with magnetic beads. Labor, time, and reagent are saved with the normalization beads.
Normalization of NGS libraries. The input NGS library amount and normalized NGS library amount are indicated.
Specifications
Specifications | |
DNA input type | NGS library, PCR, sheared genomic DNA |
DNA input range | 50-300 ng |
Normalized range | 25-60 ng |
Elution volume | > 10 µl |
Technology | Magnetic beads |
Features of DNA Normalization Beads
- Consistent normalization of various sources
- NGS libraries
- PCR fragments
- Sheared genomic DNA
- Excellent dynamic input range: 6 folds from 50 ng to 300 ng
- Magnetic beads technology
- No centrifugation
- No column
- No filtration
- Elution: as low as 10 µl without yield loss
- Removal of unwanted components and impurities
Applications of DNA Normalization Beads
- NGS library normalization
- Targeted enrichment
- PCR normalization
- Sheared genomic DNA normalization
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 40075S: 48 rxns
- Cat.# 40075L: 96 rxns