NGS Ancient DNA Library Prep Kit

The NGS Ancient DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using ancient DNA (50 ng – 500 ng) as input. The kit is based on single stranded DNA library preparation, which is more effective for ancient DNA samples. Highly degraded ancient DNA is difficult for the library preparation and usually result in low library conversion rate using regular library prep approach. It has been demonstrated by scientists that ancient DNA library prep using ssDNA library prep approach has significant better outcome than that of dsDNA library prep approach. The final library is strand specific.

NGS Ancient DNA Library Prep Kit Workflow

Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.

Comparison of library yield

Comparison of library conversion rate

Features

• Quick and simple Protocol
  • Total protocol time is only 1.5 hours
  • Hands-on time only 10 minutes
• Less magnetic beads needed: Reduced more than 50% of the beads for cleanup steps
• Directional library
• Single stranded DNA as input: From 50 ng to 500 ng

Protocol & MSDS Download

• Download link

Cat.# & Rxns

• Cat.# 30086A/30086F/30086S/30086L: 24/48/96/192 rxns

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