NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms)
The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.
NGS FFPE DNA Library Prep Kit Workflow
FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples. Â Thus, the extraction of high quality DNA from FFPE samples is often a challenge. Â Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.
Comparison of library conversion efficiency under the same condition. Input DNA amount is 25 ng.
As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.
Comparison of library yield under the same condition. Input DNA amount is 25 ng.
Library multiplexing up to 96 samples is possible with the unique dual indexes. We have developed a 4-Base Difference Index System. The system can generate indexes with at least 4 bases different from others in the 8-base indexing region. The unique dual indexing primers identify sequencing errors such as index hopping, mis-assignment, and de-multiplexing errors. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34037).
Features
- Fast protocol: 10 min of hands-on time, 1.5 hrs of  protocol time
- Less magnetic beads required for cleanup steps: Save the cost more than 50%
- Low input FFPE DNA: From 10 ng to 400 ng
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 30039A/30039F/30039S/30039L: 24/48/96/192 rxns (illumina)
- Cat.# 34037S/34037L: 24/96 rxns (MGI)
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