DNA Size Selection Kit (Magnetic Beads)
The DNA size selection ranges:
-
- 50-100 bp
- 100-200 bp
- 200-500 bp
- 250-350 bp: ideal for illumina PE100 sequencing
- 300-450 bp: ideal for illumina PE150 sequencing
- 450-750 bp: ideal for illumina PE300 sequencing
- 500-1000 bp
- 1-3 kb
- 1-5 kb
- >5 kb: ideal for long-read sequencing
- >10 kb: ideal for long-read sequencing
The series of DNA Size Selection Kits (Magnetic Beads) were developed for DNA size selection using magnetic beads. A total of 11 kits are available, with different selection ranges spanning from 50 bp to over 10 kb. The kits provide a simple and quick approach for the enrichment of a specific range of DNA fragments. The kit workflow allows double-sided or single-sided size selection for specific size cutoffs.
DNA size selection with dual clean-ups.
DNA size selection with single clean-up for >5 kb and >10 kb DNA.
DNA size selection is a selective capture of DNA fragments of a specific range of size for next-generation sequencing (NGS) library preparations, PCR, ChIP assay, DNA ligations, endonuclease digestions, adapter removal, and other genomics and molecular biology applications.
The magnetic beads technology uses paramagnetic particles, also known as SPRI (Solid Phase Reversible Immobilization) beads, to bind DNA reversibly and selectively. DNA fragments can be size-selected and purified by changing the properties of the magnetic beads or SPRI beads. The magnetic beads can easily separate the beads-binding DNA from the contaminants and unwanted components in the samples. The samples after DNA size selection are free of contaminants such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. Our proprietary magnetic beads reagents improve yield, selectivity, and reproducibility.
Gel images of different ranges of size selection. Sheared human genomic DNA was used as input.
Specific DNA fragments at a certain length range can be purified simply using magnetic separation with different beads components, avoiding tedious and time-consuming gel extraction and column-based purification. The magnetic beads method is popular for common DNA size selection, including library size selection. The first beads-binding step, referred to as the right-side clean-up, removes large DNA fragments. The large DNA fragments are bound to the beads and are discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-binding, referred to as the left-side clean-up. The double-size selected DNA fragments are eluted after ethanol rinsing.
A single clean-up is needed for DNA size selection with large fragments. In this case, only the large DNA fragments are bound to the beads. The selected larger DNA fragments are eluted after ethanol rinsing.
Features
- High specificity and high recovery of size selection
- 11 selection ranges are available, including 5 ranges for NGS library size selection
- Fast and simple
- 20-min protocol
- No gel purification required
- No columns required
- No centrifugation required
- Efficient removal of contaminants and unwanted components
Protocol & MSDS Download
Cat.# & Rxns
- Cat.# 20101S/20101L (50-100 bp): 24/96 rxns
- Cat.# 20102S/20102L (100-200 bp): 24/96 rxns
- Cat.# 20103S/20103L (200-500 bp): 24/96 rxns
- Cat.# 20104S/20104L (250-350 bp): 24/96 rxns
- Cat.# 20105S/20105L (300-450 bp): 24/96 rxns
- Cat.# 20106S/20106L (450-750 bp): 24/96 rxns
- Cat.# 20107S/20107L (500-1000 bp): 24/96 rxns
- Cat.# 20108S/20108L (1-3 kb): 24/96 rxns
- Cat.# 20109S/20109L (1-5 kb): 24/96 rxns
- Cat.# 20110S/20110L (>5 kb): 24/96 rxns
- Cat.# 20111S/20111L (>10 kb): 24/96 rxns
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