Short Oligo Quantification Kit
Long oligos (>50 nt) and ssDNA can be quantified with ssDNA Quantification kits using a Qubit Fluorometer. However, it is difficult to quantify the concentration of oligos that are shorter than 50 nt due to the inaccuracy of the data. To address the issue, we have developed the Short Oligo Quantification Kit for the quantification of oligos that are shorter than 50 nt using Qubit Fluorometer. The kit can quantify the concentration of oligos as short as 11 nt.
Comparison of oligo quantification accuracy
The kit includes Short Oligo Dye, Short Oligo Dilution Buffer, and two Standards. Simply dilute the Dye with the Dilution Buffer, add oligo sample (volume from 1 to 20 μL), then read the oligo concentration using the Qubit Fluorometer. The assay is accurate for oligo concentrations from50 pg/µL to 200 ng/µL based on the line corresponding of the data to standards.
Quantification data: 50 nt and 11 nt oligos (200, 400, 600, 800, 1000, and 1200 ng/ml) was used.
Contaminant | Concentration in sample | % of signal change |
Sodium chloride | 100 mM | 1.9% |
Calcium chloride | 1 mM | 2.9% |
Magnesium chloride | 5 mM | 1.1% |
Phenol | 0.2% | 1.0% |
Chloroform | 2% | 0.5% |
Ethanol | 10% | 1.8% |
SDS | 0.01% | 2.6% |
Triton X-100 | 0.1% | -0.8% |
Polyethylene glycerol | 1% | 1.4% |
ATP | 0.1 mM | 0.8% |
Common contaminants such as salts, solvents, or detergents are well tolerated in the assay.
Features
- Quantify the concentration of oligos from 11 to 50 nt
- Use the Qubit ssDNA assay setting
- Linear range: 1-200 ng oligo
Protocol & MSDS Download
- Cat.# 40046S; 40046L
Kit Components
- Short Oligo Dilution Buffer
- Short Oligo Dye
- Short Oligo Standard 1
- Short Oligo Standard 2
Related Products
- dsDNA Quantification Broad Range Kit (for Qubit fluorometer)
- dsDNA Quantification High Sensitivity Kit(for Qubit fluorometer)Â
- ssDNA Quantification Kit (for Qubit fluorometer)
- RNA Quantification High Sensitivity Kit (for Qubit fluorometer)
- Assay Tubes (Qubit Fluorometer)
- Assay Tube Adaptor (For Qubit Fluorometer, Half-Volume 100 µL Assay)
- dsDNA Quantification Broad Range Kit (for microplate reader)
- dsDNA Quantification High Sensitivity Kit (for microplate reader)
Note: Qubit is a registered trademark of ThermoFisher Scientific.