RNA Quantification High Sensitivity Kit
- Cat.# 40044, 200 µL Assay: standard 0.5 ml assay tubes needed
- Cat.# 40214, 100 µL Assay (half-volume): Assay tube adaptor and 100-µL assay tubes required
The RNA Quantification HS kit includes HS Dye, HS Dilution Buffer, and two RNA Standards. Simply dilute the HS Dye with the HS Dilution Buffer, add RNA sample, then read the concentration using the Qubit Fluorometer.
The assay is accurate for RNA concentrations from 250 pg/µL to 100 ng/µL. The kit has several advantages over traditional RNA quantitation such as stability, sensitivity, and contaminant tolerance.
The performance of the BioDynami RNA Quantification HS kit is nearly identical to that of Thermo Fisher’s Qubit RNA HS kit (figure above).
We have developed the half-volume Qubit assay kits with the volume of 100 µL, and the kit costs are also reduced to half of the costs for the 200 µL assay. Our kits can save 64% (standard 200 µL assay) and 80% (half-volume 100 µL assay) of the costs as compared to ThermoFisher’s kits. Plus, the multichannel pipettes and the 96-well tube racks can be used for the preparation of the 100 µL assays and the time can be reducedby 8-12 fold.
Cost comparison of RNA Quantification HS kit
Contaminant | Final concentration in the assay | Concentration in 10-μL sample | Result |
Sodium chloride | 10 mM | 200 mM | OK |
Magnesium chloride | 40 μM | 0.8 mM | OK |
Sodium acetate | 10 mM | 200 mM | OK |
Ethanol | 1% | 20% | OK |
Phenol | 0.1% | 2% | OK |
SDS | 0.005% | 0.1% | OK |
Triton X-100 | 0.001% | 0.02% | OK |
dNTP | 100 μM | 2 mM | OK |
BSA | 20 μg/mL | 400 μg/mL | OK |
DNA | 0.25X | 0.25X | OK |
Common contaminants such as detergents, solvents, salts, free nucleotides, or protein are well tolerated in the assay (Table 1).
Features
- Optimized condition for using with the Qubit Fluorometer
- Uses the Qubit RNA High Sensitivity assay setting
- Cost saving
- Save 64% (200-µL Assay)
- Save 80% (100-µL Assay)
Additional Features for 100-µL assays
- Reduced assay volume to 100 µL
- Cut the costs to 50% as compared to standard 200-µL assays
- High-throughput compatible: The 96-well plate racks and multichannel pipettes can be used.
- Shorten assay preparation time by 8-12 folds.
Protocol & MSDS Download
- Cat.# 40044SÂ and 40044L
- Cat.# 40214S and 40214L
Kit Components
- RNA HS Dilution Buffer
- RNA HS Dye
- RNA HS Standard 1
- RNA HS Standard 2
Note: Qubit is a registered trademark of ThermoFisher Scientific.
Related Products
- dsDNA Quantification Broad Range Kit (for Qubit fluorometer)
- dsDNA Quantification High Sensitivity Kit(for Qubit fluorometer)Â
- ssDNA Quantification Kit (for Qubit fluorometer)
- Short Oligo Quantification Kit (for Qubit fluorometer)
- Assay Tubes (Qubit Fluorometer)
- Assay Tube Adaptor (For Qubit Fluorometer, Half-Volume 100 µL Assay)
- dsDNA Quantification Broad Range Kit (for microplate reader)
- dsDNA Quantification High Sensitivity Kit (for microplate reader)