Magnetic Beads (DNA & RNA Purification)
Solid Phase Reversible Immobilization magnetic beads consist of paramagnetic particles coated with carboxyl groups that reversibly bind DNA. They are used for DNA purification because they are fast, simple and efficient. We have developed our own beads technology that are different from other SPRI beads technologies.
Our Magnetic Beads (DNA & RNA Purification) combines BioDynami’s proprietary chemistries with reversible DNA-binding properties of magnetic beads. The magnetic beads, which are unique from other SPRI beads, are developed for effective nucleic acid purification by removing unwanted components such as salts, dNTPs, enzymes, primers, adapters, and other impurities. The beads are RNase free, can be used for applications of DNA, and even work with more sensitive RNA without any additional cost.
Our magnetic beads are optimized to selectively bind DNA fragments of 100 bp and larger, and RNA fragments of 200 bases and larger, similar to other SPRI beads such as AMPure XP* and SPRIselect*. Purified DNA and RNA are suitable for downstream applications requiring high quality DNA and RNA, as the purified fragments are free of contaminants and impurities. The beads can be used for NGS library purification, PCR fragment cleanup, molecular cloning, or even nucleic acid concentration.
The beads can also be used for size selection of DNA fragments ranging from 150 bp to 800 bp by changing the bead-to-sample volume ratio and performing single or double-size selection. The beads are an ideal choice for NGS library preparation. They can be easily integrated into the standard workflow of NGS library preparation since the volume ratio is similar for protocols using standard magnetic beads.
Features
- Effective recovery of DNA and RNA samples
- DNA fragments >100 base pairs
- RNA fragments >200 bases
- Elution in small volume without losing yield
- As low as 10 ul is OK
- Ideal for precious, limited amount, and low-concentration samples
- Cost effective alternative to other beads such as AMPure XP and SPRIselect with equivalent performance
Magnetic beads recovery rate. dsDNA and ssDNA of genomic DNA, 1 kb DNA, and 200 bp DNA fragments were used. Total RNA was also tested.
Comparison of elution volume vs yield. 40 ul, 20 ul, 15 ul, and 10 ul of elution volumes were used. BioDynami magnetic beads have better recovery rates at low elution volume when compared to other SPRI beads such as AMPure XP. Genomic DNA and NGS library samples are used for comparison.
Applications
- NGS Library preparation
- Purification of PCR fragments
- Purification of DNA and RNA fragments
- Molecular Cloning
- Other applications requiring purified DNA and RNA
Protocol & MSDS Download
Cat.# & Volume
- Cat.# 40051S: 5 ml
- Cat.# 40051M: 20 ml
- Cat.# 40051L: 100 ml
Related Products
- Magnetic Beads (microRNA & Oligo Purification)
- Magnetic Beads (Short Oligo Purification)
- Magnetic Beads (PCR Purification)
- Magnetic Beads (tRNA Purification)
- DNA Concentrator (Magnetic Beads)
- cfDNA Purification Kit (Magnetic Beads)
- miRNA Purification Kit (tRNA Depletion)
- Plasmid Purification Magnetic Beads (RNA Depletion)
- RNA Contamination Removal Magnetic Beads (gDNA Purification)
- RNase-free RNA Removal (Magnetic Beads)
* AMPure® XP and SPRIselect are trade marks of Beckman Coulter.